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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1998-1-26
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pubmed:databankReference |
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pubmed:abstractText |
The RET proto-oncogene encodes a receptor tyrosine kinase expressed during neural crest development. RET expression is enhanced in vitro by several differentiating agents, including retinoic acid (RA), which up-regulates RET expression in neuroblastoma cell lines. In the present work we sequenced and analysed a 5 kbp genomic fragment 5' to RET. Three deletion fragments of this region were tested for their RA inducibility in transient transfection assays and failed to support the hypothesis of a direct transcriptional activation. Finally, our functional analysis of a candidate RA response element present in the RET promoter provides new hints for the understanding of the interaction between nuclear receptors and their specific recognition sites.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0014-5793
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
8
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pubmed:volume |
419
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
76-82
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:9426223-Base Sequence,
pubmed-meshheading:9426223-Drosophila Proteins,
pubmed-meshheading:9426223-Gene Expression Regulation,
pubmed-meshheading:9426223-HeLa Cells,
pubmed-meshheading:9426223-Humans,
pubmed-meshheading:9426223-Molecular Sequence Data,
pubmed-meshheading:9426223-Neuroblastoma,
pubmed-meshheading:9426223-Promoter Regions, Genetic,
pubmed-meshheading:9426223-Proto-Oncogene Proteins,
pubmed-meshheading:9426223-Proto-Oncogene Proteins c-ret,
pubmed-meshheading:9426223-Proto-Oncogenes,
pubmed-meshheading:9426223-RNA, Messenger,
pubmed-meshheading:9426223-Receptor Protein-Tyrosine Kinases,
pubmed-meshheading:9426223-Recombinant Fusion Proteins,
pubmed-meshheading:9426223-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:9426223-Restriction Mapping,
pubmed-meshheading:9426223-Sequence Analysis, DNA,
pubmed-meshheading:9426223-Transcription, Genetic,
pubmed-meshheading:9426223-Transfection,
pubmed-meshheading:9426223-Tretinoin,
pubmed-meshheading:9426223-Tumor Cells, Cultured
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pubmed:year |
1997
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pubmed:articleTitle |
Sequence and characterisation of the RET proto-oncogene 5' flanking region: analysis of retinoic acid responsiveness at the transcriptional level.
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pubmed:affiliation |
Università di Genova, Facoltà di Medicina e Laboratorio di Genetica Molecolare, Istituto G. Gaslini, Genoa, Italy. genetica@unige.it
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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