Linked Life Data

9421407

Source:http://linkedlifedata.com/resource/pubmed/id/9421407

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1998-1-15
pubmed:abstractText
The findings of the preceding article suggest that intracerebroventricular (i.c.v.) administration of small amounts (5 microl) of antisera to rats may produce effectual immunoneutralization of peptides in blood/tissues outside of the central nervous system (CNS). In the present work we sought to test this hypothesis by determining the titers of corresponding antibodies in jugular venous plasma after i.c.v. infusion of three different antisera: a sheep anti-CRF, a rabbit anti-CRF, and a rabbit anti-GnRH. For all antisera tested, corresponding antibodies were detected in systemic plasma within 30 min of i.c.v. infusion of 5 microl antiserum. By 8 h, blood levels of the corresponding antibodies were similar whether the antisera had been infused i.c.v. or i.v. When the dilutions of antibodies equivalent to those in systemic blood 1-24 h after i.c.v. infusion of 5 microl antiserum were employed in rat anterior pituitary cell culture assays, they proved effective at inhibiting CRF- or GnRH-induced hormone secretion. Furthermore, in rats pretreated i.c.v. with 5 microl anti-CRF (at -4 h), pituitary ACTH secretion induced by i.v. CRF (0.3 nmol/kg) was reduced by 88%. Collectively, these data demonstrate that shortly after i.c.v. infusion of neuropeptide antisera, the levels of corresponding antibodies found in systemic blood are sufficient to inhibit neuropeptide signaling within peripheral tissues. As i.c.v. passive immunization procedures have been used extensively in the investigation of the biological roles of neuropeptides within the CNS, these findings indicate a critical reevaluation of the peripheral vs. CNS functions of neuropeptides.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0013-7227
pubmed:author
pubmed:issnType
Print
pubmed:volume
139
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
128-36
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed:year
1998
pubmed:articleTitle
Intracerebroventricular passive immunization. II. Intracerebroventricular infusion of neuropeptide antisera can inhibit neuropeptide signaling in peripheral tissues.
pubmed:affiliation
The Clayton Foundation Laboratories for Peptide Biology, The Salk Institute, La Jolla, California 92037, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't