|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
6
|
|
pubmed:dateCreated |
1997-11-10
|
|
pubmed:abstractText |
GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1-6 fucosyltransferase (alpha1-6FucT) catalyzes the transfer of a fucosyl residue from GDP-fucose to the asparagine-linked GlcNAc residue of complex N-glycans via alpha1-6 linkage. These oligosaccharide structures are essential for the attachment of polysialic acid to the neural-cell-adhesion molecule, and its levels are useful for the differential diagnosis of hepatocellular carcinomas with respect to the microheterogeneity of alpha-fetoprotein. We have been successful in the purification and cDNA cloning of alpha1-6FucT from porcine brain and from a human gastric-cancer cell line. In the present study, mRNA expression of alpha1-6FucT in various rat tissues and human cancer cell lines was examined, along with the expression of alpha1-6FucT mRNA and the induction by treatment with several cytokines. Northern-blot analysis indicated high expression levels of alpha1-6FucT in brain and gastrointestinal-tract tissues of normal rats, as well as for a number of lung-cancer, gastric-cancer and colon-cancer cell lines. Although various cytokines did not induce alpha1-6FucT mRNA, differentiation of a tumor cell enhanced the mRNA by 2- to 3-fold. These results may provide new insight into studies on alpha1-6FucT in terms of carcinogenesis or differentiation.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Asparagine,
http://linkedlifedata.com/resource/pubmed/chemical/Fucosyltransferases,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoprotein...,
http://linkedlifedata.com/resource/pubmed/chemical/Neural Cell Adhesion Molecules,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Sialic Acids,
http://linkedlifedata.com/resource/pubmed/chemical/Tumor Markers, Biological,
http://linkedlifedata.com/resource/pubmed/chemical/polysialic acid
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Sep
|
|
pubmed:issn |
0020-7136
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
17
|
|
pubmed:volume |
72
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
1117-21
|
|
pubmed:dateRevised |
2007-7-24
|
|
pubmed:meshHeading |
pubmed-meshheading:9378548-Animals,
pubmed-meshheading:9378548-Asparagine,
pubmed-meshheading:9378548-Brain,
pubmed-meshheading:9378548-Carbohydrate Conformation,
pubmed-meshheading:9378548-Carbohydrate Sequence,
pubmed-meshheading:9378548-Carcinoma, Hepatocellular,
pubmed-meshheading:9378548-Cloning, Molecular,
pubmed-meshheading:9378548-Diagnosis, Differential,
pubmed-meshheading:9378548-Fucosyltransferases,
pubmed-meshheading:9378548-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:9378548-Humans,
pubmed-meshheading:9378548-Liver Neoplasms,
pubmed-meshheading:9378548-Male,
pubmed-meshheading:9378548-Molecular Sequence Data,
pubmed-meshheading:9378548-Neural Cell Adhesion Molecules,
pubmed-meshheading:9378548-Oligosaccharides,
pubmed-meshheading:9378548-Organ Specificity,
pubmed-meshheading:9378548-RNA, Messenger,
pubmed-meshheading:9378548-Rats,
pubmed-meshheading:9378548-Rats, Sprague-Dawley,
pubmed-meshheading:9378548-Sialic Acids,
pubmed-meshheading:9378548-Stomach Neoplasms,
pubmed-meshheading:9378548-Swine,
pubmed-meshheading:9378548-Transcription, Genetic,
pubmed-meshheading:9378548-Tumor Cells, Cultured,
pubmed-meshheading:9378548-Tumor Markers, Biological
|
|
pubmed:year |
1997
|
|
pubmed:articleTitle |
Expression of alpha1-6 fucosyltransferase in rat tissues and human cancer cell lines.
|
|
pubmed:affiliation |
Department of Biochemistry, Osaka University Medical School, Suita, Japan.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
