Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1997-11-14
pubmed:abstractText
We recently showed, using human Jurkat T cell variants lacking the T cell receptor (TCR)/CD3 complex, that the lectin jacalin is able to trigger intracellular calcium increase provided that CD4 is expressed on the cell surface. Involvement of the CD4 molecule in jacalin-induced biological effects was furthermore demonstrated in differentiated U937 myelomonocytic cells expressing or not expressing CD4, and is confirmed here in human CD4-transfected mouse thymoma cells. In the present paper, we analyze the CD4-associated calcium response triggered by jacalin independently of the TCR/CD3 complex. We show that the observed calcium rise results from a direct long-lasting calcium influx from the outside without release of calcium from intracellular stores. We demonstrate that it is independent of the phosphoinositide phospholipase C transduction pathway. Moreover, we show that this peculiar calcium response can be blocked by protein tyrosine kinase inhibitors (herbimycin and genistein) giving evidence of the involvement of a protein tyrosine kinase, the best candidate of which is the CD4-associated p56lck. Altogether, our results suggest that, independently of the TCR/CD3 complex, CD4 may be involved in the triggering of a calcium signal dependent on a protein tyrosine kinase and independent of the phosphoinositide transduction pathway.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD4, http://linkedlifedata.com/resource/pubmed/chemical/Benzoquinones, http://linkedlifedata.com/resource/pubmed/chemical/Calcium, http://linkedlifedata.com/resource/pubmed/chemical/FYN protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Fyn protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates, http://linkedlifedata.com/resource/pubmed/chemical/Isoenzymes, http://linkedlifedata.com/resource/pubmed/chemical/Lactams, Macrocyclic, http://linkedlifedata.com/resource/pubmed/chemical/Lectins, http://linkedlifedata.com/resource/pubmed/chemical/Lymphocyte Specific Protein..., http://linkedlifedata.com/resource/pubmed/chemical/Phosphatidylinositols, http://linkedlifedata.com/resource/pubmed/chemical/Phospholipase C gamma, http://linkedlifedata.com/resource/pubmed/chemical/Phosphotyrosine, http://linkedlifedata.com/resource/pubmed/chemical/Plant Lectins, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Proto-Oncogene Proteins c-fyn, http://linkedlifedata.com/resource/pubmed/chemical/Quinones, http://linkedlifedata.com/resource/pubmed/chemical/Type C Phospholipases, http://linkedlifedata.com/resource/pubmed/chemical/herbimycin, http://linkedlifedata.com/resource/pubmed/chemical/jacalin
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0014-2980
pubmed:author
pubmed:issnType
Print
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
2261-8
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:9341768-Animals, pubmed-meshheading:9341768-Antigens, CD4, pubmed-meshheading:9341768-Benzoquinones, pubmed-meshheading:9341768-CD4-Positive T-Lymphocytes, pubmed-meshheading:9341768-Calcium, pubmed-meshheading:9341768-Humans, pubmed-meshheading:9341768-Inositol Phosphates, pubmed-meshheading:9341768-Isoenzymes, pubmed-meshheading:9341768-Lactams, Macrocyclic, pubmed-meshheading:9341768-Lectins, pubmed-meshheading:9341768-Lymphocyte Specific Protein Tyrosine Kinase p56(lck), pubmed-meshheading:9341768-Mice, pubmed-meshheading:9341768-Phosphatidylinositols, pubmed-meshheading:9341768-Phospholipase C gamma, pubmed-meshheading:9341768-Phosphotyrosine, pubmed-meshheading:9341768-Plant Lectins, pubmed-meshheading:9341768-Protein-Tyrosine Kinases, pubmed-meshheading:9341768-Proto-Oncogene Proteins, pubmed-meshheading:9341768-Proto-Oncogene Proteins c-fyn, pubmed-meshheading:9341768-Quinones, pubmed-meshheading:9341768-Signal Transduction, pubmed-meshheading:9341768-Transfection, pubmed-meshheading:9341768-Tumor Cells, Cultured, pubmed-meshheading:9341768-Type C Phospholipases
pubmed:year
1997
pubmed:articleTitle
Evidence for a CD4-associated calcium influx independent of the phosphoinositide transduction pathway in human T cells.
pubmed:affiliation
Institut National de la Santé et de la Recherche Médicale (INSERM) U431, Université de Montpellier II, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't