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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2
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pubmed:dateCreated |
1997-10-22
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pubmed:abstractText |
DNA-extracts from prehistoric roots of teeth were analyzed by PCR to investigate microsatellite-systems. At the same time the x/y-chromosome specific system Amel A/B was investigated. The samples were collected from different burial conditions of similar age. Factors leading to limited DNA-degradation are given. For the first time reproducible Quadruplex-amplification on single-copy loci of prehistoric tissue was obtained in samples from the Lichtenstein-Cave, Kr. Osterode/Harz. Positive results are explained by low temperature in the burial site. In addition the results show that desiccation allows DNA-preservation but cannot protect the DNA from damage due to microbial origin. Microorganisms can destroy DNA-structures completely.
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pubmed:language |
ger
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jun
|
pubmed:issn |
0003-5548
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
55
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
193-8
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:9341086-Burial,
pubmed-meshheading:9341086-DNA,
pubmed-meshheading:9341086-History, Ancient,
pubmed-meshheading:9341086-Humans,
pubmed-meshheading:9341086-Microsatellite Repeats,
pubmed-meshheading:9341086-Paleodontology,
pubmed-meshheading:9341086-Polymerase Chain Reaction,
pubmed-meshheading:9341086-Tooth Root
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pubmed:year |
1997
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pubmed:articleTitle |
[Detection of DNA single-copy sequences of prehistoric teeth. Site milieu as a factor for preservation of DNA].
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pubmed:affiliation |
Institut für Anthropologie, Universität Göttingen.
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pubmed:publicationType |
Journal Article,
English Abstract,
Historical Article
|