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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-10-31
|
| pubmed:abstractText |
Characteristic aluminum K (AlK) (energy of 1.5 keV) and copper L (CuL) (energy of approximately 0.96 keV) ultrasoft X rays have been used to investigate the effectiveness of the numerous low-energy secondary electrons produced by low-linear energy transfer (LET) ionizing radiation. Cellular inactivation and induction and rejoining of DNA double-strand breaks (DSBs) in Chinese hamster V79-4 cells irradiated as monolayers with these ultrasoft X radiations have been studied under aerobic and anaerobic conditions. The mean cell thickness, determined by confocal laser scanning fluorescence microscopy, was used to calculate the mean dose to the nucleus of the irradiated cells. Relative to 60Co gamma rays, the relative biological effectiveness (RBE) for cellular inactivation at 10% survival is 1.7 +/- 0.1 and 2.3 +/- 0.3 for AIK and CuL ultrasoft X rays, respectively. The RBE values for induction of DSBs of 2.5 +/- 0.2 and 3.0 +/- 0.3 for AlK and CuL X rays, respectively, were determined after irradiation at 277 K using the technique of pulsed-field gel electrophoresis. Induction of DSBs is linearly dependent on dose. Oxygen enhancement ratios of 1.9 and 2.1 for cellular inactivation and DSB induction, respectively, were obtained with AIK X rays. These values are less than those for 60Co gamma radiation. The repair kinetics for rejoining of DSBs after a dose of 15 Gy is similar for both X-ray energies and 60Co gamma rays with a first half-life of 18-22 +/- 5 min. From these studies, it is suggested that induction of DSBs by low-LET radiations such as 60Co gamma rays reflects clustered damage produced predominantly by low-energy electron "track ends," which represent about 30% of the total dose.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0033-7587
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
148
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
317-24
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9339947-Aerobiosis,
pubmed-meshheading:9339947-Aluminum,
pubmed-meshheading:9339947-Anaerobiosis,
pubmed-meshheading:9339947-Animals,
pubmed-meshheading:9339947-Cell Line,
pubmed-meshheading:9339947-Chromosomes,
pubmed-meshheading:9339947-Copper,
pubmed-meshheading:9339947-Cricetinae,
pubmed-meshheading:9339947-Cricetulus,
pubmed-meshheading:9339947-DNA,
pubmed-meshheading:9339947-DNA Damage,
pubmed-meshheading:9339947-DNA Repair,
pubmed-meshheading:9339947-Dose-Response Relationship, Radiation,
pubmed-meshheading:9339947-Fibroblasts,
pubmed-meshheading:9339947-Gamma Rays,
pubmed-meshheading:9339947-Linear Energy Transfer,
pubmed-meshheading:9339947-Oxygen,
pubmed-meshheading:9339947-Relative Biological Effectiveness,
pubmed-meshheading:9339947-X-Rays
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Induction and rejoining of DNA double-strand breaks in Chinese hamster V79-4 cells irradiated with characteristic aluminum K and copper L ultrasoft X rays.
|
| pubmed:affiliation |
MRC Radiation and Genome Stability Unit, Didcot, Oxfordshire, United Kingdom.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|