Linked Life Data

9323368

Source:http://linkedlifedata.com/resource/pubmed/id/9323368

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
6
pubmed:dateCreated
1997-11-6
pubmed:abstractText
Following binding and internalization into the host cell cytoplasm, elementary bodies (EB) of the obligate intracellular bacterium Chlamydia trachomatis undergo a developmental process resulting in production of reticulate bodies (RB), the vegetative growth form of the organism. EB are metabolically inactive, but EB to RB transformation requires bacterial protein synthesis. Using HeLa cells infected with EB of C. trachomatis serovar C, we examined the time of first appearance of transcripts from several genes whose products are required for assembly and function of the chlamydial protein synthetic system. We monitored appearance of chlamydial RNAs using reverse transcription-polymerase chain reaction assays targeting primary transcripts from the bacterial rRNA operons, and mRNAs encoding the glycyl tRNA synthetase and the ribosomal proteins S5 and L5. Transcripts from the proximal rRNA promoters, and those from the r-protein and tRNA synthetase genes, are detectable as early as 4 h after EB-host binding; transcripts from distal rRNA promoters do not appear until 6 h post-infection. Thus, expression of bacterial genes whose products are required for protein synthesis begins earlier in chlamydial EB to RB development than previously thought.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0026-8925
pubmed:author
pubmed:issnType
Print
pubmed:volume
255
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
637-42
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Genes required for assembly and function of the protein synthetic system in Chlamydia trachomatis are expressed early in elementary to reticulate body transformation.
pubmed:affiliation
Department of Microbiology and Immunology, Allegheny University of the Health Sciences, MCP Hahnemann School of Medicine, Philadelphia, PA 19129, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't