Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
4
|
| pubmed:dateCreated |
1997-10-23
|
| pubmed:abstractText |
Liver regeneration is a physiological mechanism that leads to restoration of the hepatic parenchyma following hepatectomy or toxic injury. As a two-third hepatectomy is the usual model to analyse this phenomenon, few studies have compared liver regeneration after minor vs major hepatectomy. We have used a quantitative RT-PCR technique to study the hepatic transcription of the TGF-alpha gene in rats submitted to 30% or 80% hepatectomy and we have correlated this transcription with the regenerative response assessed by flow cytometry and Ki-67 expression. The level of TGF-alpha expression and the regenerative response were different, according to the volume of liver removed, and were statistically correlated (r = 0.679, p = 0.002). TGF-alpha expression and phase S peaked at day 2 vs 6 in the 80% vs 30% hepatectomized rat groups, respectively. Ki-67 expression occurred at 2 h post-hepatectomy in the two groups of rats and was observed until day 14 mainly in the 80% hepatectomy group. Our results indicate that TGF-alpha expression in regenerating liver is strongly correlated with hepatocyte mitosis, that a delayed regenerative response occurs following 30% hepatectomy and that the course of regeneration differs between minor vs major hepatectomy.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0106-9543
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
17
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
171-6
|
| pubmed:dateRevised |
2003-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:9298486-Animals,
pubmed-meshheading:9298486-DNA Primers,
pubmed-meshheading:9298486-DNA Replication,
pubmed-meshheading:9298486-Flow Cytometry,
pubmed-meshheading:9298486-Gene Expression,
pubmed-meshheading:9298486-Hepatectomy,
pubmed-meshheading:9298486-Ki-67 Antigen,
pubmed-meshheading:9298486-Liver,
pubmed-meshheading:9298486-Liver Regeneration,
pubmed-meshheading:9298486-Male,
pubmed-meshheading:9298486-Polymerase Chain Reaction,
pubmed-meshheading:9298486-RNA, Messenger,
pubmed-meshheading:9298486-Rats,
pubmed-meshheading:9298486-Rats, Sprague-Dawley,
pubmed-meshheading:9298486-Regression Analysis,
pubmed-meshheading:9298486-S Phase,
pubmed-meshheading:9298486-Transcription, Genetic,
pubmed-meshheading:9298486-Transforming Growth Factor alpha
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Transforming growth factor alpha (TGF-alpha) expression correlates with DNA replication in regenerating rat liver whatever the hepatectomy extent.
|
| pubmed:affiliation |
INSERM U 78 and Institut Fédératif de Recherche Multidisciplinaire sur les Peptides Bois-Guillaume, France.
|
| pubmed:publicationType |
Journal Article
|