Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1997-9-30
|
| pubmed:abstractText |
OCI-5, the rat homologue of human glypican 3 (GPC3), is believed to be involved in morphogenesis and growth control during development. The finding that GPC3 is mutated in patients with the Simpson-Golabi-Behmel overgrowth syndrome is consistent with this idea. In this report, using RNA in situ hybridization, expression of OCI-5 in the developing intestine is detected in both endoderm- and mesenchyme-derived cells in a phased manner related to age and proximal/distal position. To investigate the mechanism of its regulation during intestinal development, OCI-5 expression was studied in the primitive rat intestinal epithelial cell line IEC-18. The expression of the OCI-5 transcript is increased in IEC-18 cells at confluence, in low calcium media, and during spheroid culture, all conditions which result in the cells acquiring a more rounded cell shape. In contrast, cytoskeletal disruption with colchicine causes cells to flatten and spread and abolishes both the confluence- and the low calcium-dependent induction of OCI-5. Treatment with vanadate, a phosphatase inhibitor, causes cells to acquire a spindle-shaped morphology and prevents OCI-5 induction in all situations. Nuclear run-on analysis demonstrates that the rate of OCI-5 transcription is increased at confluence, in low calcium media, and during spheroid culture of IEC-18, and decreased by treatment of cells with colchicine. Together, these data suggest that OCI-5 expression is regulated in IEC-18 by cell shape. The pattern of expression of OCI-5 in the developing intestine is consistent with it playing a role in epithelial-mesenchymal interactions during intestinal morphogenesis, when cell shape changes are likely to occur.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Chloride,
http://linkedlifedata.com/resource/pubmed/chemical/Glypicans,
http://linkedlifedata.com/resource/pubmed/chemical/Gpc3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Heparan Sulfate Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Heparitin Sulfate,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Proteoglycans,
http://linkedlifedata.com/resource/pubmed/chemical/Vanadates
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Aug
|
| pubmed:issn |
0014-4827
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
25
|
| pubmed:volume |
235
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3-12
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9281346-Animals,
pubmed-meshheading:9281346-Calcium Chloride,
pubmed-meshheading:9281346-Cell Line,
pubmed-meshheading:9281346-Cell Nucleus,
pubmed-meshheading:9281346-Colon,
pubmed-meshheading:9281346-Duodenum,
pubmed-meshheading:9281346-Gene Expression Regulation, Developmental,
pubmed-meshheading:9281346-Glypicans,
pubmed-meshheading:9281346-Heparan Sulfate Proteoglycans,
pubmed-meshheading:9281346-Heparitin Sulfate,
pubmed-meshheading:9281346-Humans,
pubmed-meshheading:9281346-Intestinal Mucosa,
pubmed-meshheading:9281346-Kinetics,
pubmed-meshheading:9281346-Membrane Proteins,
pubmed-meshheading:9281346-Morphogenesis,
pubmed-meshheading:9281346-Proteoglycans,
pubmed-meshheading:9281346-Rats,
pubmed-meshheading:9281346-Rats, Sprague-Dawley,
pubmed-meshheading:9281346-Transcription, Genetic,
pubmed-meshheading:9281346-Vanadates
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Expression of OCI-5/glypican 3 during intestinal morphogenesis: regulation by cell shape in intestinal epithelial cells.
|
| pubmed:affiliation |
Ontario Cancer Institute/Princess Margaret Hospital, Toronto, Ontario, Canada.
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|