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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
34
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pubmed:dateCreated |
1997-9-15
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pubmed:abstractText |
The POU domain transcription factor Brn-3a is able to stimulate neurite outgrowth when overexpressed in the neuronal ND7 cell line, whereas the closely related Brn-3b factor does not have this effect. We show that Brn-3a overexpression also enhances the expression of the three neurofilament genes at both the mRNA and protein levels, whereas Brn-3b overexpression has no effect. In addition Brn-3a activates the three neurofilament gene promoters in co-transfection assays in both neuronal and non-neuronal cells. As observed for enhanced neurite outgrowth, the stimulation of neurofilament gene expression and activation of the neurofilament gene promoters is observed with the isolated POU domain of Brn-3a. A single amino acid change in the POU homeodomain of Brn-3a to the equivalent amino acid in Brn-3b abolishes its ability to activate the neurofilament promoters, whereas the reciprocal change converts Brn-3b to an activator of these promoters.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0021-9258
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
22
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pubmed:volume |
272
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
21325-33
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:9261145-Animals,
pubmed-meshheading:9261145-Binding Sites,
pubmed-meshheading:9261145-Cell Line,
pubmed-meshheading:9261145-DNA-Binding Proteins,
pubmed-meshheading:9261145-Gene Expression Regulation,
pubmed-meshheading:9261145-Hybrid Cells,
pubmed-meshheading:9261145-Mice,
pubmed-meshheading:9261145-Neurofilament Proteins,
pubmed-meshheading:9261145-Neurons,
pubmed-meshheading:9261145-Promoter Regions, Genetic,
pubmed-meshheading:9261145-RNA, Messenger,
pubmed-meshheading:9261145-Rats,
pubmed-meshheading:9261145-Sequence Deletion,
pubmed-meshheading:9261145-Structure-Activity Relationship,
pubmed-meshheading:9261145-Transcription, Genetic,
pubmed-meshheading:9261145-Transcription Factor Brn-3,
pubmed-meshheading:9261145-Transcription Factor Brn-3A,
pubmed-meshheading:9261145-Transcription Factor Brn-3B,
pubmed-meshheading:9261145-Transcription Factors
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pubmed:year |
1997
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pubmed:articleTitle |
Coordinate induction of the three neurofilament genes by the Brn-3a transcription factor.
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pubmed:affiliation |
Department of Molecular Pathology, University College London Medical School, London W1P 6DB, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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