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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1997-8-25
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pubmed:abstractText |
It is essential for the study of T-cell function and the improvement of adoptive cell therapies to efficiently generate large populations of human primary T cells that reliably express foreign genes. This goal is achieved by using recombinant retroviruses pseudotyped with either the gibbon ape leukemia virus (GaLV) envelope or the vesicular stomatitis virus G (VSV-G) glycoprotein. We show here that both retroviral particles mediate stable gene transfer in CD4+ and in CD8+ peripheral blood lymphocytes cultured under optimized conditions. However, VSV-G-pseudotyped virions may cause transduction artifacts that must be carefully excluded. The VSV-G virions require 10- to 100-fold higher concentrations of infectious particles to achieve levels of gene transfer comparable to GaLV-virions. Nonetheless, the physical stability of VSV-G-coated particles enables the concentration of viral stocks to 10(9) infectious particles per milliliter or more.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/G protein, vesicular stomatitis...,
http://linkedlifedata.com/resource/pubmed/chemical/Gene Products, env,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Nerve Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-4971
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
952-7
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:9242523-3T3 Cells,
pubmed-meshheading:9242523-Animals,
pubmed-meshheading:9242523-Artifacts,
pubmed-meshheading:9242523-CD4-Positive T-Lymphocytes,
pubmed-meshheading:9242523-CD8-Positive T-Lymphocytes,
pubmed-meshheading:9242523-DNA, Complementary,
pubmed-meshheading:9242523-False Positive Reactions,
pubmed-meshheading:9242523-Gene Products, env,
pubmed-meshheading:9242523-Genetic Vectors,
pubmed-meshheading:9242523-HeLa Cells,
pubmed-meshheading:9242523-Humans,
pubmed-meshheading:9242523-Leukemia Virus, Gibbon Ape,
pubmed-meshheading:9242523-Lung Neoplasms,
pubmed-meshheading:9242523-Membrane Glycoproteins,
pubmed-meshheading:9242523-Mice,
pubmed-meshheading:9242523-Receptor, Nerve Growth Factor,
pubmed-meshheading:9242523-Receptors, Nerve Growth Factor,
pubmed-meshheading:9242523-Recombinant Fusion Proteins,
pubmed-meshheading:9242523-Transduction, Genetic,
pubmed-meshheading:9242523-Transfection,
pubmed-meshheading:9242523-Tumor Cells, Cultured,
pubmed-meshheading:9242523-Vesicular stomatitis Indiana virus,
pubmed-meshheading:9242523-Viral Envelope Proteins
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pubmed:year |
1997
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pubmed:articleTitle |
Recombinant retroviruses pseudotyped with the vesicular stomatitis virus G glycoprotein mediate both stable gene transfer and pseudotransduction in human peripheral blood lymphocytes.
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pubmed:affiliation |
Department of Human Genetics, Memorial Sloan-Kettering Cancer Center, New York, NY 10021, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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