Linked Life Data

9241807

Source:http://linkedlifedata.com/resource/pubmed/id/9241807

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-9-3
pubmed:abstractText
An effective detection system for TBEV-RNA sequences using a RT-PCR technique has been developed. In our system, specific oligonucleotide primers corresponding to the 5'-terminal noncoding region were successfully used to identify TBEV sequences in ticks. To prove the specificity of the PCR products, Southern blot hybridization with an internal digoxigenin-labelled probe was carried out. In this paper, we present some potential applications of this technique. The primers were used to identify 21 TBEV strains isolated in different years, in different geographic regions and from different sources. 22313 Ixodes ricinus ticks from north-east Germany were analyzed for TBEV-specific sequences in order to characterize the viral activity in natural foci of TBE. In the new Federal Länder, only 6 samples gave positive PCR-results, showing that the natural foci of TBE had not been extinguished but remained in a state of endemic latency. We also used the RT-PCR to develop an animal model to investigate the temporal pattern of viraemia in the Mongolian gerbil (Meriones unguiculatus) through xenodiagnosis (sequential tick feeding on an infected host and subsequent RT-PCR testing of the resultant engorged ticks).
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jun
pubmed:issn
0934-8840
pubmed:author
pubmed:issnType
Print
pubmed:volume
286
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
125-38
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Tick-borne encephalitis virus (TBEV)-specific RT-PCR for characterization of natural foci of TBE and for other applications.
pubmed:affiliation
Federal Institute for Health Protection of Consumers and Veterinary Medicine, Berlin, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't