Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1997-7-31
pubmed:abstractText
We have identified an open reading frame (ORF), CP530R, within the EcoRI C' fragment of the African swine fever virus (ASFV) genome that encodes a polyprotein of 62 kDa (pp62). Antisera raised against different regions of ORF CP530R recognized a polypeptide of 62 kDa in ASFV-infected cells during the late phase of virus replication, after the onset of viral DNA synthesis. Pulse-chase experiments showed that polyprotein pp62 is posttranslationally processed to give rise to two proteins of 35 kDa (p35) and 15 kDa (p15). This proteolytic processing was found to take place at the consensus sequence Gly-Gly-X through an ordered cascade of proteolytic cleavages like that which also occurs with ASFV polyprotein pp220 (C. Simón-Mateo, G. Andrés, and E. Viñuela, EMBO J. 12:2977-2987, 1993). Immunofluorescence studies showed that polyprotein pp62 is localized in the viral factories. In addition, immunoprecipitation analysis of purified virus particles showed that mature products p35 and p15 are major structural proteins. According to these results, polyprotein processing represents an essential strategy for the maturation of ASFV structural proteins.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-11831707, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-1317266, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-1740191, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-2199796, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-2722819, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-3012867, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-3052288, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-3763393, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-3776350, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-3882998, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-3989907, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-500712, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-5269234, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-5432063, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-6324451, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-6324453, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-6546423, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-6695498, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-7355576, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-7853518, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-7871730, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-823294, http://linkedlifedata.com/resource/pubmed/commentcorrection/9223468-8335009
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0022-538X
pubmed:author
pubmed:issnType
Print
pubmed:volume
71
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5799-804
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Proteolytic processing in African swine fever virus: evidence for a new structural polyprotein, pp62.
pubmed:affiliation
Centro de Biología Molecular Severo Ochoa (Consejo Superior de Investigaciones Cientificas-Universidad Autónoma de Madrid), Facultad de Ciencias, Universidad Autonoma de Madrid, Cantoblanco, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't