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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1997-8-4
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pubmed:abstractText |
The cellulase complex from Trichoderma reesei was polyethylene glycol (PEG)-modified with considerable retention of endo-beta-1,4-glucanase activity, as evaluated by the carboxymethylcellulase (CMCase) assay. While resistance towards heat denaturation was the same for either form, susceptibility towards proteolysis was slightly greater for the PEG-conjugate, in contrast to most reports using other enzymes. The circulatory life of endoglucanase activity associated with the complex was enhanced upon PEG-modification. This was confirmed by demonstrating degradation of chromogen-labelled substrate injected (i.v.) after 24hr of administration of the PEG-ylated enzymes; in contrast the substrate remained undegraded in mice pretreated with the native complex. The PEG-modified complex could be a good candidate for the treatment of pneumoconioses of textile workers exposed to cotton dust.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Jun
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pubmed:issn |
1039-9712
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
42
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
93-102
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pubmed:dateRevised |
2010-11-18
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pubmed:meshHeading |
pubmed-meshheading:9192089-Animals,
pubmed-meshheading:9192089-Blotting, Western,
pubmed-meshheading:9192089-Carboxymethylcellulose Sodium,
pubmed-meshheading:9192089-Catalysis,
pubmed-meshheading:9192089-Cellulase,
pubmed-meshheading:9192089-Coloring Agents,
pubmed-meshheading:9192089-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:9192089-Glycoside Hydrolases,
pubmed-meshheading:9192089-Hot Temperature,
pubmed-meshheading:9192089-Hydrogen-Ion Concentration,
pubmed-meshheading:9192089-Male,
pubmed-meshheading:9192089-Mice,
pubmed-meshheading:9192089-Mice, Inbred BALB C,
pubmed-meshheading:9192089-Polyethylene Glycols,
pubmed-meshheading:9192089-Protein Denaturation,
pubmed-meshheading:9192089-Trichoderma
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pubmed:year |
1997
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pubmed:articleTitle |
Enhanced in vivo catalytic activity of PEG-modified cellulase complex from Trichoderma reesei.
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pubmed:affiliation |
Department of Biochemistry, University of Delhi, India. bic_dusc@ernet.in
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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