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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
10
|
| pubmed:dateCreated |
1997-6-19
|
| pubmed:abstractText |
We used a stroma-supported culture method to study the prevalence and growth characteristics of malignant stem cells in acute lymphoblastic leukemia (ALL). In 51 of 108 B-lineage ALL samples, bone marrow-derived stroma not only inhibited apoptosis of ALL cells but also supported their proliferation in serum-free medium. When single leukemic cells were placed in the stroma-coated wells of microtiter plates, the percentage of wells with leukemic cell growth after 2 to 5 months of culture ranged from 6% to 20% (median, 15%; 5 experiments). The immunophenotypes and genetic features of cells recovered from these cultures were identical to those noted before culture. All cells maintained their stroma dependency and self-renewal capacity. Leukemic clones derived from single cells contained approximately 10(3) to 10(6) cells after 1 month of culture; other clones became detectable only after prolonged culture. Cell growth in stroma-coated wells correlated with the number of initially seeded cells (1 or 10; r = .87). However, the observed percentages of positive wells seeded with 10 cells always exceeded values predicted from results with single-cell-initiated cultures (P < .003 by paired t-test), suggesting stimulation of leukemic cell growth by paracrine factors. In conclusion, the proportion of ALL cells with clonogenic potential may be considerably higher than previously thought.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD19,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Neoplasm,
http://linkedlifedata.com/resource/pubmed/chemical/Culture Media, Serum-Free,
http://linkedlifedata.com/resource/pubmed/chemical/HLA-DR Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-7,
http://linkedlifedata.com/resource/pubmed/chemical/Stem Cell Factor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
May
|
| pubmed:issn |
0006-4971
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
|
| pubmed:volume |
89
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
3735-44
|
| pubmed:dateRevised |
2007-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:9160679-Adipose Tissue,
pubmed-meshheading:9160679-Antigens, CD19,
pubmed-meshheading:9160679-Antigens, Neoplasm,
pubmed-meshheading:9160679-Bone Marrow Cells,
pubmed-meshheading:9160679-Burkitt Lymphoma,
pubmed-meshheading:9160679-Cell Division,
pubmed-meshheading:9160679-Child,
pubmed-meshheading:9160679-Coculture Techniques,
pubmed-meshheading:9160679-Connective Tissue,
pubmed-meshheading:9160679-Connective Tissue Cells,
pubmed-meshheading:9160679-Culture Media, Serum-Free,
pubmed-meshheading:9160679-HLA-DR Antigens,
pubmed-meshheading:9160679-Humans,
pubmed-meshheading:9160679-Immunophenotyping,
pubmed-meshheading:9160679-Interleukin-7,
pubmed-meshheading:9160679-Neoplastic Stem Cells,
pubmed-meshheading:9160679-Stem Cell Factor,
pubmed-meshheading:9160679-Tumor Cells, Cultured,
pubmed-meshheading:9160679-Tumor Stem Cell Assay
|
| pubmed:year |
1997
|
| pubmed:articleTitle |
Prevalence and growth characteristics of malignant stem cells in B-lineage acute lymphoblastic leukemia.
|
| pubmed:affiliation |
Department of Hematology-Oncology, St Jude Children's Research Hospital, University of Tennessee College of Medicine, Memphis 38105, USA.
|
| pubmed:publicationType |
Journal Article
|