Linked Life Data

9155731

Source:http://linkedlifedata.com/resource/pubmed/id/9155731

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-6-12
pubmed:abstractText
The molecular basis for the recruitment of maternal mRNAs after fertilization is poorly understood, since there is little information available regarding the identity of such mRNAs. An assay based on reverse transcriptase and PCR was used to identify a maternal mRNA that undergoes a transient polyadenylation after fertilization; the length of the poly(A) tail increases from 40-80 adenosine (A) residues in the unfertilized egg to approximately 250 3 h after insemination and then decreases to about 180 around the time of pronucleus formation, that is 7 h after insemination. The DNA sequence of this cDNA, which encodes the polyadenylation signal AAUAA, contains a uridine-rich sequence that may serve as the cytoplasmic polyadenylation element observed in other maternal mRNAs that are recruited after fertilization.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Mar
pubmed:issn
0022-4251
pubmed:author
pubmed:issnType
Print
pubmed:volume
109
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
223-8
pubmed:dateRevised
2007-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
Transient polyadenylation of a maternal mRNA following fertilization of mouse eggs.
pubmed:affiliation
Department of Biology, University of Pennsylvania, Philadelphia 19104-6018, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't