Linked Life Data

9135042

Source:http://linkedlifedata.com/resource/pubmed/id/9135042

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
3
pubmed:dateCreated
1997-7-21
pubmed:abstractText
Whole-cell patch-clamp recordings were made from neurons in the substantia gelatinosa of adult rat spinal cord slices with attached dorsal root to study a slow synaptic current evoked by focal or dorsal root stimulation. Repetitive focal stimulation with a monopolar electrode positioned within substantia gelatinosa elicited a slow excitatory postsynaptic current preceded by a fast excitatory postsynaptic current in 73 of 83 neurons. A similar slow excitatory postsynaptic current was also elicited by stimulation of A delta afferent fibres. The amplitude of slow excitatory postsynaptic currents was unchanged when the recording electrode contained guanosine-5'-O-(2-thiodiphosphate). The slow excitatory postsynaptic current and current evoked by aspartate revealed similar reversal potentials and showed a marked outward rectification at holding potentials more negative than -30 mV, while the glutamate-induced current exhibited a relatively linear voltage relationship. In addition, the slow excitatory postsynaptic currents were reversibly occluded during the aspartate-induced current but were not occluded during the glutamate-induced current. The slow excitatory postsynaptic currents evoked by focal stimulation were depressed but not abolished by 6-cyano-7-nitroquinoxaline-2,3-dione (10 microM) or by 6-cyano-7-nitroquinoxaline-2,3-dione together with DL-2-amino-5-phosphonopentanoic acid (100 microM). Similarly, the aspartate- and glutamate-induced currents were also resistant to these antagonists. These observations suggest that a transmitter released from interneurons or descending fibres which are activated in part by A delta afferents, mediates a slow excitatory postsynaptic currents in substantia gelatinosa neurons and that an excitatory amino acid is implicated in the generation of the slow excitatory postsynaptic current, although the receptor appears to differ from the known ligand-gated channels. C afferents are unlikely to contribute to the slow excitatory postsynaptic current. This slow synaptic response may participate in the pain pathway and play an important role in the processing of nociceptive information in the spinal dorsal horn.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0306-4522
pubmed:author
pubmed:issnType
Print
pubmed:volume
76
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
673-88
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
A novel slow excitatory postsynaptic current in substantia gelatinosa neurons of the rat spinal cord in vitro.
pubmed:affiliation
Department of Physiology, Kurume University School of Medicine, Japan.
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't