Linked Life Data

9078469

Source:http://linkedlifedata.com/resource/pubmed/id/9078469

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1997-6-26
pubmed:abstractText
Restriction endonuclease site maps were constructed for the genome of a caprine adenovirus (GAdV), strain NC90-7261, which was isolated in 1990 from a 3-year-old goat with encephalitis. Genomic GAdV DNA was digested with seven restriction endonucleases (RE). Genomic DNA libraries of GAdV were constructed by cloning BamHI and HindIII restriction fragments into a plasmid vector. Using cloned GAdV genomic fragments as probes in Southern blot hybridizations, an RE site map was constructed. The position of several clones was confirmed by limited nucleotide sequencing and the location of several RE sites was determined by single or double RE digestions of cloned fragments. The size of the GAdV genome was determined to be 28.2 kbp. The restriction pattern described in this report is different from that of other adenoviruses. Although the genomic organization of this GAdV is likely to be similar to that of other adenoviruses, the overall level of sequence similarity is low.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0300-5526
pubmed:author
pubmed:issnType
Print
pubmed:volume
39
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
275-9
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Molecular characterization of a caprine adenovirus.
pubmed:affiliation
Department of Microbiology, School of Veterinary Medicine, Tuskegee University, Ala, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't