Linked Life Data

9042407

Source:http://linkedlifedata.com/resource/pubmed/id/9042407

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1997-3-21
pubmed:abstractText
K-ras gene sequences mutant at codon 12 were recovered following differential dot-blot hybridization of genomic DNA from human RSa cells up to 12 days after the cells had been irradiated with far-UV (principally 254 nm). By contrast, no mutant codon 12 sequences were recovered from cells which had been treated with 50 IU/ml human interferon (HuIFN)-alpha for 24 h prior to their UV exposure. HuIFN-alpha treatment in combination with anti-HuIFN-alpha antibody did not lead to the loss of mutant sequences. However, culture of interferon-pretreated cells with medium containing the protease inhibitor antipain (0.01 mM) for 6 h immediately after UV irradiation led to the recovery of mutant codon 12 sequences. Thus, while treatment with HuIFN-alpha appeared to prevent any UV-induced mutations affecting codon 12 of the K-ras gene from being recovered, the putative antipain-sensitive protease responsible for this suppressive affect appeared to be significantly affected by the protease inhibitor antipain.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Feb
pubmed:issn
0027-5107
pubmed:author
pubmed:issnType
Print
pubmed:day
3
pubmed:volume
373
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
251-6
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed:year
1997
pubmed:articleTitle
UV-induced mutations affecting codon 12 of the K-ras gene are suppressed by interferon-alpha in human RSa cells.
pubmed:affiliation
Department of Biochemistry, School of Medicine, Chiba University, Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't