Linked Life Data

8997524

Source:http://linkedlifedata.com/resource/pubmed/id/8997524

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
8
pubmed:dateCreated
1997-6-19
pubmed:abstractText
Some proteases (particularly elastase) and metabolites of very reactive oxygen species (superoxide anion, hydrogen peroxide, hypochlorous acid and hydroxyl radicals) are generated by polymorphonuclear neutrophils (PMNs) during inflammatory disorders. Divalent cations, especially calcium (Ca2+) play an important regulatory role in the different PMNs functions. The aim of this study is to determine the role of extracellular calcium during the liberation of elastase and of reactive oxygen species production by human PMNs. Consequently, in order to stimulate PMNs, phorbolmyristate acetate (PMA), formyl-methionyl-leucyl-phenylalanine (fMLP) and opsonized zymosan (OZ) have been used. PMNs stimulated by OZ did not release elastase to reverse the PMA and fMLP systems. The production of elastase by PMNs stimulated by PMA to reverse the fMLP system is independent from the extracellular calcium, between 0.0 and 1.5 mM. With various higher concentrations of calcium, varying from 1.5 to 4.0 mM, the release of elastase by PMNs stimulated by PMA is extracellular calcium-dependent to reverse the fMLP system. The superoxide anion (O2-) generated by PMNs activated by fMLP is dependent from the extracellular calcium in the medium, whereas O2- production by PMA or OZ stimulated neutrophils was extracellular calcium-independent. These observations suggest that an influx of calcium may play an important role in the production of elastase and in the capacity of PMNs stimulated by fMLP to produce O2- to reverse the PMA and OZ systems.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0145-5680
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1211-8
pubmed:dateRevised
2005-11-17
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Involvement of the extracellular calcium in the release of elastase and the human neutrophils oxidative burst.
pubmed:affiliation
Laboratoire de Pharmacologie, Pharmacocinétique Clinique, Faculté des Sciences, Pharmaceutiques et Biologiques, Lille, France.
pubmed:publicationType
Journal Article