Linked Life Data

8977181

Source:http://linkedlifedata.com/resource/pubmed/id/8977181

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1997-1-30
pubmed:abstractText
Culture of day 14 mouse fetal liver (FL) cells in high dose IL-2, together with appropriate combinations of IL-4 and PMA, resulted in the generation of cell lines, termed FL-A lines, that were phenotypically and functionally indistinguishable from cultured adult splenic NK cell populations with the single important exception that no Ly49-expressing cells were present. By contrast, when FL cells were cultured in low-dose IL-2 alone, a second population of slow-growing NK-like cells, termed FL-B cells, emerged. These cells expressed the NK markers asialoGM1, 10A7, 2B4, and Fc gammaRII/III but differed from FL-A and splenic NK cells in expressing IL-2R alpha and stem cell factor receptor (SCFR) but no B220. Most lines derived in this manner had minimal or no cytolytic activity and only very low levels of NK1.1. However, they could secrete substantial quantities of several lymphokines including IL-3, granulocyte-macrophage (GM)-CSF, TNF-alpha, and, most surprisingly, IL-2. A minority of FL-B lines, typified by line 903, displayed marked cytolytic activity, moderate levels of NK1.1, reduced production of IL-2, and the capacity for accelerated growth in high-dose IL-2. FL-B lines generally expressed mRNA for CD3gamma but not for other CD3 chains, whereas FL-A and fetal thymic (FT) NK lines often expressed mRNA for all four CD3 chains. Despite many similarities to pro-T cells, FL-B cells showed no capacity to differentiate into mature T cells. Taken together, our results suggest that NK lines of different maturity can be obtained from fetal liver, with FL-B lines being the most immature, FL-A lines the most mature, and lines such as FL-B 903 representing an intermediate state of differentiation.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Jan
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
158
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
112-9
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:8977181-Animals, pubmed-meshheading:8977181-Antigens, CD3, pubmed-meshheading:8977181-Antigens, Ly, pubmed-meshheading:8977181-Antigens, Surface, pubmed-meshheading:8977181-Cell Differentiation, pubmed-meshheading:8977181-Cell Line, pubmed-meshheading:8977181-Culture Techniques, pubmed-meshheading:8977181-Embryonic and Fetal Development, pubmed-meshheading:8977181-Interleukin-2, pubmed-meshheading:8977181-Interleukin-4, pubmed-meshheading:8977181-Killer Cells, Natural, pubmed-meshheading:8977181-Lectins, C-Type, pubmed-meshheading:8977181-Liver, pubmed-meshheading:8977181-Lymphokines, pubmed-meshheading:8977181-Membrane Glycoproteins, pubmed-meshheading:8977181-Mice, pubmed-meshheading:8977181-Mice, Inbred BALB C, pubmed-meshheading:8977181-Mice, Inbred C57BL, pubmed-meshheading:8977181-Mice, Nude, pubmed-meshheading:8977181-RNA, Messenger, pubmed-meshheading:8977181-Receptors, NK Cell Lectin-Like, pubmed-meshheading:8977181-Tetradecanoylphorbol Acetate
pubmed:year
1997
pubmed:articleTitle
Phenotypic and functional characterization of long-lived NK cell lines of different maturational status obtained from mouse fetal liver.
pubmed:affiliation
Department of Immunology, Medical School, University of Newcastle, Newcastle upon Tyne, United Kingdom.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't