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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1997-3-5
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pubmed:abstractText |
In this study we systematically assessed the sensitivity limits of fiber-FISH in model experiments. Exonic fragments and cDNAs with exon sizes of >/=200 bp could be mapped on their cognate cosmid. This positional fiber-FISH mapping was validated by long-range PCR. It is expected that these two independent mapping approaches will help to refine current available gene maps and show their applicability in fine mapping of sequence-tagged sites or expressed sequence tags. Also, they will be useful in resolving gene structures by mapping exon and intron locations.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0888-7543
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
38
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
277-82
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8975703-Chromosome Mapping,
pubmed-meshheading:8975703-Cosmids,
pubmed-meshheading:8975703-DNA, Complementary,
pubmed-meshheading:8975703-DNA, Recombinant,
pubmed-meshheading:8975703-Dystrophin,
pubmed-meshheading:8975703-Exons,
pubmed-meshheading:8975703-Humans,
pubmed-meshheading:8975703-In Situ Hybridization, Fluorescence,
pubmed-meshheading:8975703-Microscopy, Fluorescence,
pubmed-meshheading:8975703-Muscular Dystrophies,
pubmed-meshheading:8975703-Polymerase Chain Reaction,
pubmed-meshheading:8975703-Sensitivity and Specificity,
pubmed-meshheading:8975703-Sequence Tagged Sites
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pubmed:year |
1996
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pubmed:articleTitle |
Exon mapping by fiber-FISH or LR-PCR.
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pubmed:affiliation |
Department of Cytochemistry and Cytometry, State University Leiden, Wassenaarseweg 72, Leiden, 2333 AL, The Netherlands.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
|