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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1-2
|
| pubmed:dateCreated |
1997-1-7
|
| pubmed:databankReference | |
| pubmed:abstractText |
We report the isolation and characterization of genomic sequences coding for the 5' end of human retinoic acid receptor beta 1, a fetal RAR isoform which is also expressed in small cell lung cancers. Primer extension analysis revealed a principal transcription start site with a secondary site 23 bp further upstream in both SCLC and fetal tissues. The sequences isolated were CpG-rich between -60 and the 3' end of the first exon but there were no features like a TATA-box or an Inr element. In several important aspects, the human RAR beta 1 differs from its mouse counterpart. The 5'-UTR is 823 bp long and is encoded by four exons (vs two in the mouse giving a 477 bp UTR). The 5' end of the mouse cDNA has been mapped to a 67 bp CT repeat which is absent in our sequences. We were also unable to find evidence for a human isoform RAR beta 3 which is widely expressed in mouse tissues.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0006-3002
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
11
|
| pubmed:volume |
1309
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1-4
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8950166-Animals,
pubmed-meshheading:8950166-Base Sequence,
pubmed-meshheading:8950166-Carcinoma, Small Cell,
pubmed-meshheading:8950166-Cloning, Molecular,
pubmed-meshheading:8950166-CpG Islands,
pubmed-meshheading:8950166-DNA, Complementary,
pubmed-meshheading:8950166-Dinucleotide Repeats,
pubmed-meshheading:8950166-Exons,
pubmed-meshheading:8950166-Genes,
pubmed-meshheading:8950166-Humans,
pubmed-meshheading:8950166-Lung Neoplasms,
pubmed-meshheading:8950166-Mice,
pubmed-meshheading:8950166-Molecular Sequence Data,
pubmed-meshheading:8950166-RNA, Messenger,
pubmed-meshheading:8950166-Receptors, Retinoic Acid,
pubmed-meshheading:8950166-Sequence Analysis, DNA,
pubmed-meshheading:8950166-Sequence Homology, Nucleic Acid,
pubmed-meshheading:8950166-Transcription, Genetic,
pubmed-meshheading:8950166-Tumor Cells, Cultured
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Structure of the human retinoic acid receptor beta 1 gene.
|
| pubmed:affiliation |
Institut du Cancer de Montréal, Centre de Recherche Louis-Charles Simard, Montréal, Canada. toulousa@ere.umontreal.ca
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|