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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1997-1-7
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pubmed:abstractText |
The switch from membrane-bound to secreted-form IgM that occurs during differentiation of B lymphocytes has long been known to involve regulated processing of the heavy chain pre-mRNA. Here, we show that accumulation of one subunit of an essential polyadenylation factor (CstF-64) is specifically repressed in mouse primary B cells and that overexpression of CstF-64 is sufficient to switch heavy chain expression from membrane-bound (microm) to secreted form (micros). We further show that CstF-64 is limiting for formation of intact CstF, that CstF has a higher affinity for the microm poly(A) site than for the micros site, and that the microm site is stronger in a reconstituted in vitro processing reaction. Our results indicate that CstF-64 plays a key role in regulating IgM heavy chain expression during B cell differentiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0092-8674
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
29
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pubmed:volume |
87
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
941-52
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8945520-Alternative Splicing,
pubmed-meshheading:8945520-Animals,
pubmed-meshheading:8945520-B-Lymphocytes,
pubmed-meshheading:8945520-Cell Differentiation,
pubmed-meshheading:8945520-Cell Line,
pubmed-meshheading:8945520-Cell Membrane,
pubmed-meshheading:8945520-Chickens,
pubmed-meshheading:8945520-Gene Expression Regulation,
pubmed-meshheading:8945520-Immunoglobulin Heavy Chains,
pubmed-meshheading:8945520-Immunoglobulin M,
pubmed-meshheading:8945520-Mice,
pubmed-meshheading:8945520-Mice, Inbred C3H,
pubmed-meshheading:8945520-Mice, Inbred C57BL,
pubmed-meshheading:8945520-RNA, Messenger,
pubmed-meshheading:8945520-RNA Precursors,
pubmed-meshheading:8945520-RNA-Binding Proteins,
pubmed-meshheading:8945520-Rabbits,
pubmed-meshheading:8945520-mRNA Cleavage and Polyadenylation Factors
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pubmed:year |
1996
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pubmed:articleTitle |
The polyadenylation factor CstF-64 regulates alternative processing of IgM heavy chain pre-mRNA during B cell differentiation.
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pubmed:affiliation |
Department of Biological Sciences, Columbia University, New York, New York 10027, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
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