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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-12-20
|
| pubmed:databankReference | |
| pubmed:abstractText |
The recent cloning of several cDNAs encoding prostaglandin (PG) receptors has paved the way for a more detailed investigation of the postulated regulatory role of prostaglandins in corpus luteum function. We have utilized the reverse transcription-polymerase chain reaction (RT-PCR) to isolate a mRNA encoding the ovarian PGF(2alpha) (FP) receptor, using oligonucleotides based on the recently cloned mouse cDNA as primers. The 5'-untranslated region of the rat ovarian mRNA was isolated following 5'-RACE (rapid amplification of cDNA ends). The isolated 1526 base-pair sequence, which spans the entire open reading frame, was found 100% identical in the protein coding region to a similar sequence isolated from a rat astrocyte cDNA library, but different in the first 32 nucleotides of the 5'-untranslated region, possibly due to tissue-specific splicing heterogeneity. Using ribonuclease protection assay, a quantitative analysis of FP receptor mRNA levels was performed in corpora lutea excised from adult pseudopregnant rats (Day 8) at different timepoints (0.5-48 h) following the in vivo s.c. regimen of a luteolytic dose of the FP receptor agonist cloprostenol (5 microg). Already 3 h after cloprostenol injection, FP receptor mRNA levels exhibited a pronounced increase to values 4.0-fold higher (P < 0.01) than before injection. At 7 h through 24 h, the amount of luteal FP receptor mRNA decreased, approaching preinjection levels, whereafter they were again 3.0-fold higher (P < 0.01) at 48 h than before injection. We conclude that following homologous stimulation of the FP receptor, abundance of this mRNA is tissue-specifically regulated in a dynamic pattern, suggestive of an important role for FP receptor-mediated action on gene expression during the demise of corpus luteum function.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Cloprostenol,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/Dinoprost,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Prostaglandin,
http://linkedlifedata.com/resource/pubmed/chemical/prostaglandin F2alpha receptor
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
|
| pubmed:issn |
0303-7207
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
14
|
| pubmed:volume |
123
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
45-52
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:8912810-Amino Acid Sequence,
pubmed-meshheading:8912810-Animals,
pubmed-meshheading:8912810-Base Sequence,
pubmed-meshheading:8912810-Cloning, Molecular,
pubmed-meshheading:8912810-Cloprostenol,
pubmed-meshheading:8912810-Corpus Luteum,
pubmed-meshheading:8912810-DNA, Complementary,
pubmed-meshheading:8912810-DNA Primers,
pubmed-meshheading:8912810-Dinoprost,
pubmed-meshheading:8912810-Female,
pubmed-meshheading:8912810-Mice,
pubmed-meshheading:8912810-Molecular Sequence Data,
pubmed-meshheading:8912810-Ovary,
pubmed-meshheading:8912810-Polymerase Chain Reaction,
pubmed-meshheading:8912810-RNA, Messenger,
pubmed-meshheading:8912810-Rats,
pubmed-meshheading:8912810-Rats, Sprague-Dawley,
pubmed-meshheading:8912810-Receptors, Prostaglandin,
pubmed-meshheading:8912810-Transcription, Genetic
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Characterization and regulation of a mRNA encoding the prostaglandin F2alpha receptor in the rat ovary.
|
| pubmed:affiliation |
Department of Physiology, University of Umeå, Sweden. jan.olofsson@obstgyn.umu.se
|
| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|