Linked Life Data

8899988

Source:http://linkedlifedata.com/resource/pubmed/id/8899988

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1997-1-8
pubmed:abstractText
The efficacy of the mitochondrially encoded cytochrome b gene as a molecular marker for the discrimination of the reservoir host species of the Lyme borreliosis spirochete, Borrelia burgdorferi sensu lato (s.l.), in its European vector Ixodes ricinus (Acari: Ixodidae) was determined. Degenerate PCR primers were designed which amplified orthologous regions of the cytochrome b gene in several animal species which act as B. burgdorferi s.l. reservoirs and hosts for I. ricinus. PCR products were amplified and characterized by hybridization and restriction fragment length polymorphism analysis. Restriction fragment length polymorphism analysis of a 638-bp PCR product with HaeIII and DdeI revealed unique restriction fragment profiles, which allowed the taxonomic identification of animals to the genus level. A system was devised for the detection of the larval host blood meal from the remnants in unfed nymphal I. ricinus ticks by nested PCR amplification. An inverse correlation was demonstrated between amplicon size and successful PCR amplification of host DNA from the nymphal stage of the tick. The stability of the cytochrome b product as a marker for the identification of the larval host species in the nymphal instar was demonstrated up to 200 days after larval ingestion (approximately 165 days after molting) by reverse line blotting with a host-specific probe. This assay has the potential for the determination of the reservoir hosts of B. burgdorferi s.l. by using extracts from the same individual ticks for both the identification of the host species and the detection of the Lyme borreliosis spirochete.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-1195397, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-1460621, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-15463419, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-1856296, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-1901092, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-2656708, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-2862839, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-3186445, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-3397814, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-3785382, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-7120390, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-7332926, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-8039659, http://linkedlifedata.com/resource/pubmed/commentcorrection/8899988-8586679
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0099-2240
pubmed:author
pubmed:issnType
Print
pubmed:volume
62
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4060-5
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
A molecular marker for the identification of the zoonotic reservoirs of Lyme borreliosis by analysis of the blood meal in its European vector Ixodes ricinus.
pubmed:affiliation
Environmental Resource Management, University College Dublin, Belfield, Ireland.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't