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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1
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pubmed:dateCreated |
1996-12-24
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pubmed:abstractText |
Porcine brain microvascular endothelial cells (PBMEC) were isolated from fresh brains by several enzymatic digestion steps followed by a gradient centrifugation. Cells of the primary culture were transfected with pRNS-1, encoding for the small and large T-antigens of SV 40, by means of lipofection. After selection with G-418, clones were isolated and one clone, PBMEC/C1-2, was further characterized by microscopic examination of morphology, immunofluorescence, and lectin binding. PBMEC/C1-2 was cultivated for nearly 1 year with 250 cumulative population doublings and showed the typical morphology of capillary endothelial cells in vitro. Postconfluent cultures showed the formation of a tubular network as a second layer, which is characteristic of capillary endothelial cells. SV 40 T-antigens were present in the nuclei and the cells showed the typical granular staining of von Willebrand factor (vWF) and were positive for Bandeiraea simplicifolia isolectin B4. Furthermore, PBMEC/C1-2 exhibited the uptake of acetylated LDL, expression of nonmuscular- and smooth-muscle-type myosins, and the presence of the blood-brain barrier-associated markers gamma-glutamyltranspeptidase (gamma-GT), the glucose transporter Glut-1, and apolipoprotein A-1. In addition, enzymatic activity of gamma-GT and alkaline phosphatase could be detected in cell suspensions. In summary, PBMEC/C1-2 represents an immortalized PBMEC line exhibiting endothelial characteristics as well as typical markers of the blood-brain barrier.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Alkaline Phosphatase,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Polyomavirus Transforming,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Lipoproteins, LDL,
http://linkedlifedata.com/resource/pubmed/chemical/acetyl-LDL,
http://linkedlifedata.com/resource/pubmed/chemical/gamma-Glutamyltransferase,
http://linkedlifedata.com/resource/pubmed/chemical/von Willebrand Factor
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0014-4827
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
10
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pubmed:volume |
228
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
50-7
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pubmed:dateRevised |
2004-11-17
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pubmed:meshHeading |
pubmed-meshheading:8892970-Alkaline Phosphatase,
pubmed-meshheading:8892970-Animals,
pubmed-meshheading:8892970-Antigens, Polyomavirus Transforming,
pubmed-meshheading:8892970-Blood-Brain Barrier,
pubmed-meshheading:8892970-Brain,
pubmed-meshheading:8892970-Cell Culture Techniques,
pubmed-meshheading:8892970-Cell Line,
pubmed-meshheading:8892970-Cell Separation,
pubmed-meshheading:8892970-Clone Cells,
pubmed-meshheading:8892970-Endothelium, Vascular,
pubmed-meshheading:8892970-Lectins,
pubmed-meshheading:8892970-Lipoproteins, LDL,
pubmed-meshheading:8892970-Microcirculation,
pubmed-meshheading:8892970-Swine,
pubmed-meshheading:8892970-Transfection,
pubmed-meshheading:8892970-gamma-Glutamyltransferase,
pubmed-meshheading:8892970-von Willebrand Factor
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pubmed:year |
1996
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pubmed:articleTitle |
Establishment of the permanent microvascular endothelial cell line PBMEC/C1-2 from porcine brains.
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pubmed:affiliation |
Institut für Biochemie, Technische Hochschule Darmstadt, Germany.
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pubmed:publicationType |
Journal Article
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