| pubmed-article:8892686 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8892686 | lifeskim:mentions | umls-concept:C0143630 | lld:lifeskim |
| pubmed-article:8892686 | lifeskim:mentions | umls-concept:C2323499 | lld:lifeskim |
| pubmed-article:8892686 | lifeskim:mentions | umls-concept:C1167622 | lld:lifeskim |
| pubmed-article:8892686 | lifeskim:mentions | umls-concept:C1998793 | lld:lifeskim |
| pubmed-article:8892686 | lifeskim:mentions | umls-concept:C1705938 | lld:lifeskim |
| pubmed-article:8892686 | lifeskim:mentions | umls-concept:C1527178 | lld:lifeskim |
| pubmed-article:8892686 | pubmed:issue | 11 | lld:pubmed |
| pubmed-article:8892686 | pubmed:dateCreated | 1996-12-3 | lld:pubmed |
| pubmed-article:8892686 | pubmed:abstractText | To characterize Kit expressing mouse bone marrow (BM) cells, and to determine their contribution to short- and long-term repopulation of the hemopoietic system of irradiated recipients, we have purified Kit+ BM cells by flow cytometry. A high level of Kit expression was detectable on 1-2% of BM cells after staining with biologically active biotinylated Kit ligand (KL) or with anti-Kit antibodies (ACK-2). Compared to unfractionated BM, the Kit+ fractions were enriched for immature hemopoietic cells, as shown by morphological differentiation, in vitro culture, and spleen colony formation. Enrichment of colony-forming cells was higher in biotin-KL+ than ACK-2+ fractions. Colony-forming cells were not found in the Kit- subsets. To study the hemopoietic repopulation capacity of the Kit+ and Kit- cells, serial dilutions of the sorted fractions were transplanted into irradiated mice, and peripheral blood of these recipients was monitored regularly for the presence of donor-derived cells during a 1 year period. Nucleated blood cell repopulation by male donor cells in female recipients was assessed using a Y-chromosome specific DNA probe; erythroid repopulation by normal donor cells in W/Wv recipients was examined flow cytometrically by measuring the forward light scatter of donor- and host-type erythrocytes. A 25- to 100-fold enrichment of long-term repopulating ability in the sorted Kit+ fractions showed that Kit+ cells are capable of reconstitution of circulating erythrocytes and nucleated blood cells after BM transplantation. Transient repopulation of the red blood cell lineage was observed after transplantation of Kit- cells. Detection of donor-derived nucleated cells 1 year after transplantation showed that Kit+ cells contributed to donor-type repopulation of bone marrow, spleen and thymus. Our data demonstrate that isolation of BM cells on the basis of Kit expression is a useful addition to the methods that are commonly applied in stem cell enrichment protocols. | lld:pubmed |
| pubmed-article:8892686 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8892686 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892686 | pubmed:citationSubset | IM | lld:pubmed |
| pubmed-article:8892686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892686 | pubmed:chemical | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8892686 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8892686 | pubmed:month | Nov | lld:pubmed |
| pubmed-article:8892686 | pubmed:issn | 0887-6924 | lld:pubmed |
| pubmed-article:8892686 | pubmed:author | pubmed-author:VisserJ WJW | lld:pubmed |
| pubmed-article:8892686 | pubmed:author | pubmed-author:WagemakerGG | lld:pubmed |
| pubmed-article:8892686 | pubmed:author | pubmed-author:de JongM OMO | lld:pubmed |
| pubmed-article:8892686 | pubmed:author | pubmed-author:RozemullerHH | lld:pubmed |
| pubmed-article:8892686 | pubmed:author | pubmed-author:WognumA WAW | lld:pubmed |
| pubmed-article:8892686 | pubmed:author | pubmed-author:KieboomDD | lld:pubmed |
| pubmed-article:8892686 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8892686 | pubmed:volume | 10 | lld:pubmed |
| pubmed-article:8892686 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8892686 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8892686 | pubmed:pagination | 1813-22 | lld:pubmed |
| pubmed-article:8892686 | pubmed:dateRevised | 2006-11-15 | lld:pubmed |
| pubmed-article:8892686 | pubmed:meshHeading | pubmed-meshheading:8892686-... | lld:pubmed |
| pubmed-article:8892686 | pubmed:meshHeading | pubmed-meshheading:8892686-... | lld:pubmed |
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| pubmed-article:8892686 | pubmed:meshHeading | pubmed-meshheading:8892686-... | lld:pubmed |
| pubmed-article:8892686 | pubmed:meshHeading | pubmed-meshheading:8892686-... | lld:pubmed |
| pubmed-article:8892686 | pubmed:meshHeading | pubmed-meshheading:8892686-... | lld:pubmed |
| pubmed-article:8892686 | pubmed:year | 1996 | lld:pubmed |
| pubmed-article:8892686 | pubmed:articleTitle | Purification of repopulating hemopoietic cells based on binding of biotinylated Kit ligand. | lld:pubmed |
| pubmed-article:8892686 | pubmed:affiliation | Institute of Hematology, Erasmus University Rotterdam, The Netherlands. | lld:pubmed |
| pubmed-article:8892686 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8892686 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
