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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
5
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pubmed:dateCreated |
1997-2-3
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pubmed:abstractText |
Serum cystatin C concentration correlates negatively with glomerular filtration rate as well as or better than that of serum creatinine, suggesting a constant formation, and elimination from extracellular fluid mainly by glomerular filtration. It is not known, however, how well the renal plasma clearance of this 13-kDa basic polypeptide matches the glomerular filtration rate. This was investigated in rats during control conditions and after reduced renal perfusion pressure. 125I-cystatin C and an indicator for glomerular filtration (51Cr-EDTA or 131I-aprotinin) were injected intravenously. The renal accumulation and urinary excretion of the tracers were recorded in periods of 2.5 to 20.0 min. The renal plasma clearance of 125I-cystatin C (Ccy) based on the renal content of 125I correlated well with the glomerular filtration rate (CCr-EDTA) in periods up to 6 min; i.e. Ccy = 0.94 x CCr-EDTA, r = 0.99. Less than 0.5% of the filtered amount appeared in the urine. During more prolonged periods, Ccy increasingly underestimated glomerular filtration rate, reaching about 0.4 x CCr-EDTA in a 20-min period. Free 125I relative to total plasma 125I activity increased from about 2% at 5 min to about 70% at 20 min. In nephrectomized rats, free 125I accumulated in plasma at a slower rate, accounting for about 15% of the total activity 20 min after injection of 125I-cystatin C. We conclude that cystatin C is (a) mainly removed from the extracellular fluid by the kidneys, (b) practically freely filtered in the glomeruli, and (c) completely absorbed and rapidly broken down by the proximal tubular cells.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Aprotinin,
http://linkedlifedata.com/resource/pubmed/chemical/CST3 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chromium Radioisotopes,
http://linkedlifedata.com/resource/pubmed/chemical/Cst3 protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Cystatin C,
http://linkedlifedata.com/resource/pubmed/chemical/Cystatins,
http://linkedlifedata.com/resource/pubmed/chemical/Cysteine Proteinase Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Edetic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Iodine Radioisotopes
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0036-5513
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
56
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
409-14
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8869663-Animals,
pubmed-meshheading:8869663-Aprotinin,
pubmed-meshheading:8869663-Chromium Radioisotopes,
pubmed-meshheading:8869663-Cystatin C,
pubmed-meshheading:8869663-Cystatins,
pubmed-meshheading:8869663-Cysteine Proteinase Inhibitors,
pubmed-meshheading:8869663-Edetic Acid,
pubmed-meshheading:8869663-Female,
pubmed-meshheading:8869663-Glomerular Filtration Rate,
pubmed-meshheading:8869663-Humans,
pubmed-meshheading:8869663-Iodine Radioisotopes,
pubmed-meshheading:8869663-Kidney,
pubmed-meshheading:8869663-Rats,
pubmed-meshheading:8869663-Rats, Sprague-Dawley
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pubmed:year |
1996
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pubmed:articleTitle |
Renal handling of radiolabelled human cystatin C in the rat.
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pubmed:affiliation |
Department of Physiology, University of Bergen, Norway.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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