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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1996-11-4
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pubmed:abstractText |
Delivery of antigen in a manner that induces effective, antigen-specific immunity is a critical challenge in vaccine design. Optimal antigen presentation is mediated by professional antigen-presenting cells (APCs) capable of taking up, processing and presenting antigen to T cells in the context of costimulatory signals required for T-cell activation. Developing immunization strategies to optimize antigen presentation by dendritic cells, the most potent APCs, is a rational approach to vaccine design. Here we show that cutaneous genetic immunization with naked DNA results in potent, antigen-specific, cytotoxic T lymphocyte-mediated protective tumor immunity. This method of immunization results in the transfection of skin-derived dendritic cells, which localize in the draining lymph nodes. These observations provide a basis for further development of DNA-based vaccines and demonstrate the feasibility of genetically engineering dendritic cells in vivo.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1078-8956
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
2
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1122-8
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8837611-Amino Acid Sequence,
pubmed-meshheading:8837611-Animals,
pubmed-meshheading:8837611-Antigen Presentation,
pubmed-meshheading:8837611-Biolistics,
pubmed-meshheading:8837611-Cell Movement,
pubmed-meshheading:8837611-Dendritic Cells,
pubmed-meshheading:8837611-Epitopes,
pubmed-meshheading:8837611-Feasibility Studies,
pubmed-meshheading:8837611-Female,
pubmed-meshheading:8837611-H-2 Antigens,
pubmed-meshheading:8837611-Immunization,
pubmed-meshheading:8837611-Lymph Nodes,
pubmed-meshheading:8837611-Lymphoma, T-Cell,
pubmed-meshheading:8837611-Melanoma, Experimental,
pubmed-meshheading:8837611-Mice,
pubmed-meshheading:8837611-Mice, Inbred C57BL,
pubmed-meshheading:8837611-Molecular Sequence Data,
pubmed-meshheading:8837611-Neoplasm Transplantation,
pubmed-meshheading:8837611-Ovalbumin,
pubmed-meshheading:8837611-T-Lymphocytes, Cytotoxic,
pubmed-meshheading:8837611-Tumor Cells, Cultured,
pubmed-meshheading:8837611-Vaccines, DNA
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pubmed:year |
1996
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pubmed:articleTitle |
DNA-based immunization by in vivo transfection of dendritic cells.
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pubmed:affiliation |
Department of Dermatology, University of Pittsburgh School of Medicine, Pennsylvania 15213, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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