8807288

umls-concept:C0017262, umls-concept:C0017337, umls-concept:C0020792, umls-concept:C0036025, umls-concept:C0185117, umls-concept:C0204727, umls-concept:C0205409, umls-concept:C1412236, umls-concept:C2911684

1997-2-6

Two cis-acting elements have been identified that act synergistically to regulate expression of the glucose-repressed alcohol dehydrogenase 2 (ADH2) gene. UAS1 is bound by the trans-activator Adr1p. UAS2 is thought to be the binding site for an unidentified regulatory protein. A genetic selection based on a UAS2-dependent ADH2 reporter was devised to isolate genes capable of activating UAS2-dependent transcription. One set of UAS2-dependent genes contained SPT6/CRE2/SSN20. Multicopy SPT6 caused improper expression of chromosomal ADH2. A second set of UAS2-dependent clones contained a previously uncharacterized open reading frame designated MEU1 (Multicopy Enhancer of UAS2). A frame shift mutation in MEU1 abolished its ability to activate UAS2-dependent gene expression. Multicopy MEU1 expression suppressed the constitutive ADH2 expression caused by cre2-1. Disruption of MEU1 reduced endogenous ADH2 expression about twofold but had no effect on cell viability or growth. No homologues of MEU1 were identified by low-stringency Southern hybridization of yeast genomic DNA, and no significant homologues were found in the sequence data bases. A MEU1/beta-gal fusion protein was not localized to a particular region of the cell. MEU1 is linked to PPR1 on chromosome XII.

http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1310793, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1312673, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1330335, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1332230, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1355328, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1427033, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1597176, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1848010, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1882550, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1899286, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-1923769, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2161531, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2176269, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2407614, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2644045, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2648133, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2659436, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2834270, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2835632, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-2852814, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3029564, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3160930, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3290644, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3290650, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3302603, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3333305, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3537711, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3547080, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-3900928, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-392242, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-6096561, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-6336730, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-6345791, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-6373495, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-6392016, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-7031263, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-7760841, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-8083216, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-8196627, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-8321238, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-8441413, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-8514151, http://linkedlifedata.com/resource/pubmed/commentcorrection/8807288-8670846

http://linkedlifedata.com/resource/pubmed/journal/0374636

http://linkedlifedata.com/resource/pubmed/chemical/Alcohol Dehydrogenase, http://linkedlifedata.com/resource/pubmed/chemical/Aldose-Ketose Isomerases, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary, http://linkedlifedata.com/resource/pubmed/chemical/DNA, Fungal, http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Glucose, http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/SPT6 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins, http://linkedlifedata.com/resource/pubmed/chemical/TRP1 protein, S cerevisiae, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcriptional Elongation Factors, http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase

Jul

pubmed-author:DonovielM SMS, pubmed-author:YoungE TET

143

Y

2011-11-17