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rdf:type |
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lifeskim:mentions |
umls-concept:C0006675,
umls-concept:C0007600,
umls-concept:C0028778,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0034826,
umls-concept:C0178719,
umls-concept:C0205263,
umls-concept:C0439799,
umls-concept:C0702240,
umls-concept:C1514758
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pubmed:issue |
18
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pubmed:dateCreated |
1996-10-24
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pubmed:abstractText |
We have studied the properties of r-eag voltage-activated potassium channels in a stably transfected human embryonic kidney cell line. It was found that r-eag channels are rapidly and reversibly inhibited by a rise in intracellular calcium from 30 to 300 nM. The inhibition does not appear to depend on the activity of calcium-dependent kinases and phosphatases. The effect of calcium on r-eag channel activity was studied in inside-out membrane patches. Calcium inhibited r-eag channel activity with a mean IC50 of 67 nM. Activation of muscarinic receptors, generating calcium oscillations in the transfected cells, induced a synchronous inhibition of r-eag mediated outward currents. This shows that calcium can mediate r-eag current inhibition following muscarinic receptor activation. The data indicate that r-eag channels are calcium-inhibitable voltage-activated potassium channels.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-1319996,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-1742024,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-1840699,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-1846449,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-1905146,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-1947178,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-2072734,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-3670292,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-6770893,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-7522674,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-7604285,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-7692301,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-7736582,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-7925287,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-8072541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-8159766,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-8207467,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-8254522,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-8261514,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-8562079,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-8662307,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8790430-8866863
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
3
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pubmed:volume |
93
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
9910-4
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8790430-Animals,
pubmed-meshheading:8790430-Calcium,
pubmed-meshheading:8790430-Cell Line,
pubmed-meshheading:8790430-Electrophysiology,
pubmed-meshheading:8790430-Ether-A-Go-Go Potassium Channels,
pubmed-meshheading:8790430-Humans,
pubmed-meshheading:8790430-Ionomycin,
pubmed-meshheading:8790430-Kidney,
pubmed-meshheading:8790430-Potassium Channels,
pubmed-meshheading:8790430-Rats,
pubmed-meshheading:8790430-Receptors, Muscarinic,
pubmed-meshheading:8790430-Spectrometry, Fluorescence,
pubmed-meshheading:8790430-Transfection
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pubmed:year |
1996
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pubmed:articleTitle |
Elevation of intracellular calcium by muscarinic receptor activation induces a block of voltage-activated rat ether-à-go-go channels in a stably transfected cell line.
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pubmed:affiliation |
Zentrum für Moleculare Neurobiologie, Universität Hamburg, Germany.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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