Linked Life Data

8770180

Source:http://linkedlifedata.com/resource/pubmed/id/8770180

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2 Pt 2
pubmed:dateCreated
1996-10-30
pubmed:databankReference
pubmed:abstractText
The capacity to concentrate urine develops progressively during postnatal life in most mammalian species. Here we have examined whether low expression of the arginine vasopressin (AVP)-activated water channel aquaporin-2 (AQP-2) may be a limiting factor for the concentrating capacity in the infant rats. Urine osmolality in response to 24-h dehydration increased significantly from 10 to 40 days of age. The most rapid increase occurred during the weaning period, i.e., days 15-20. A similar developmental pattern was observed for AQP-2 mRNA levels in the renal medulla. AQP-2 protein levels also increased markedly from day 10 to 40. Immunohistochemistry revealed that AQP-2 was exclusively located in collecting duct principal cells both in infant and adult rats but that the signal was much weaker in infants. To further examine the relationship between urinary concentrating capacity and AQP-2 expression, we treated rats with a single injection of betamethasone, which is known to accelerate maturation in several organs. Twenty-four hours after treatment, there was an increase in urine osmolality, renal medullary AQP-2 mRNA, and AQP-2 protein levels in infant but not in adult rats. A single injection of a specific V2 agonist caused within 6 h significant increase of AQP-2 mRNA in both infant and adult. The expression of the mRNA of three other transporters involved in the concentrating process, medullary Na(+)-K(+)-ATPase alpha-subunit, Na-K-2Cl cotransporter, and epithelial chloride channel also increased during the weaning period and were upregulated by glucocorticoids. We conclude that there is a well-synchronized development of the many of the components that determine the concentrating capacity and that the low expression of AQP-2 is one of the limiting factors for low concentrating capacity in infants.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Aug
pubmed:issn
0002-9513
pubmed:author
pubmed:issnType
Print
pubmed:volume
271
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
F461-8
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:8770180-Aging, pubmed-meshheading:8770180-Animals, pubmed-meshheading:8770180-Aquaporin 2, pubmed-meshheading:8770180-Aquaporin 6, pubmed-meshheading:8770180-Aquaporins, pubmed-meshheading:8770180-Base Sequence, pubmed-meshheading:8770180-Betamethasone, pubmed-meshheading:8770180-Blotting, Northern, pubmed-meshheading:8770180-Deamino Arginine Vasopressin, pubmed-meshheading:8770180-Immunohistochemistry, pubmed-meshheading:8770180-Ion Channels, pubmed-meshheading:8770180-Kidney Concentrating Ability, pubmed-meshheading:8770180-Molecular Probes, pubmed-meshheading:8770180-Molecular Sequence Data, pubmed-meshheading:8770180-Polymerase Chain Reaction, pubmed-meshheading:8770180-RNA, Messenger, pubmed-meshheading:8770180-Rats, pubmed-meshheading:8770180-Rats, Sprague-Dawley, pubmed-meshheading:8770180-Renal Agents, pubmed-meshheading:8770180-Transcription, Genetic
pubmed:year
1996
pubmed:articleTitle
Development of urinary concentrating capacity: role of aquaporin-2.
pubmed:affiliation
Department of Woman and Child Health, St. Göran's Children's Hospital, Karolinska Institute, Stockholm, Sweden.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't