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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1996-9-25
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pubmed:databankReference |
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pubmed:abstractText |
We cloned the full-length rat leptin receptor (Ob-R) isoform complementary DNAs (cDNAs) and examined the gene expression in rats. We also identified a mutation in Ob-R in Zucker fatty (fa/fa) rats. Three alternatively spliced isoforms (Ob-Ra, Ob-Rb, and Ob-Re) have been identified, which are closely related to the gp130 signal-transduction component of class I cytokine receptors. Rat Ob-Ra and Ob-Rb were single transmembrane proteins, which differ in the C-terminal amino acid sequences. On the other hand, Ob-Re had no transmembrane domain and was a soluble form of the receptor. Reverse transcription-polymerase chain reaction analysis revealed that Ob-R isoform messenger RNAs (mRNAs) are expressed in a wide variety of rat tissues in tissue-specific manners. A missense mutation (an A to C conversion at nucleotide position 806) was found in the extracellular domain of all the isoforms in Zucker fatty (fa/fa) rats, which resulted in an amino acid change from Gln to Pro at + 269 (the Gln269Pro mutation). These Ob-R isoform mRNAs were present in the brain from Zucker fatty (fa/fa) rats at comparable amounts to those in their lean littermates. The present study provides new insight into the molecular mechanisms for Ob-R.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cell Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Cytokine,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Leptin,
http://linkedlifedata.com/resource/pubmed/chemical/leptin receptor, human,
http://linkedlifedata.com/resource/pubmed/chemical/leptin receptor, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
5
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pubmed:volume |
225
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
75-83
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:8769097-Alternative Splicing,
pubmed-meshheading:8769097-Amino Acid Sequence,
pubmed-meshheading:8769097-Animals,
pubmed-meshheading:8769097-Base Sequence,
pubmed-meshheading:8769097-Carrier Proteins,
pubmed-meshheading:8769097-Cloning, Molecular,
pubmed-meshheading:8769097-DNA, Complementary,
pubmed-meshheading:8769097-DNA Primers,
pubmed-meshheading:8769097-Gene Expression,
pubmed-meshheading:8769097-Humans,
pubmed-meshheading:8769097-Male,
pubmed-meshheading:8769097-Mice,
pubmed-meshheading:8769097-Mice, Obese,
pubmed-meshheading:8769097-Molecular Sequence Data,
pubmed-meshheading:8769097-Obesity,
pubmed-meshheading:8769097-Organ Specificity,
pubmed-meshheading:8769097-Point Mutation,
pubmed-meshheading:8769097-Polymerase Chain Reaction,
pubmed-meshheading:8769097-RNA, Messenger,
pubmed-meshheading:8769097-Rats,
pubmed-meshheading:8769097-Rats, Sprague-Dawley,
pubmed-meshheading:8769097-Rats, Zucker,
pubmed-meshheading:8769097-Receptors, Cell Surface,
pubmed-meshheading:8769097-Receptors, Cytokine,
pubmed-meshheading:8769097-Receptors, Leptin,
pubmed-meshheading:8769097-Sequence Homology, Amino Acid
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pubmed:year |
1996
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pubmed:articleTitle |
Molecular cloning of rat leptin receptor isoform complementary DNAs--identification of a missense mutation in Zucker fatty (fa/fa) rats.
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pubmed:affiliation |
Department of Medicine and Clinical Science, Kyoto University Graduate School of Medicine, Japan.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, Non-U.S. Gov't
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