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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
2-3
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pubmed:dateCreated |
1996-10-15
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pubmed:databankReference | |
pubmed:abstractText |
A Gram-negative sporulating thermophilic anaerobe, designated AB11Ad, was isolated from the heated waters of the Great Artesian Basin of Australia. It grew on a variety of carbohydrates including glucose, starch, and dextran and produced a thermostable and thermoactive extracellular endo-dextranase. The enzyme was produced more actively under pH controlled continuous culture conditions than under batch conditions. Ammonium sulfate precipitated crude dextranase exhibited a temperature optimum of 70 degrees C and a pH optimum between 5 and 6. The half life was approximately 6.5 h at 75 degrees C and 2 h at 80 degrees C at pH 5.0 and in the absence of added dextran. 16S rRNA sequence analysis indicated that isolate AB11Ad was a member of the genus Thermoanaerobacter.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Jul
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pubmed:issn |
0378-1097
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
140
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
271-6
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8764490-Australia,
pubmed-meshheading:8764490-Bacteria, Anaerobic,
pubmed-meshheading:8764490-Carbohydrate Metabolism,
pubmed-meshheading:8764490-Dextranase,
pubmed-meshheading:8764490-Enzyme Stability,
pubmed-meshheading:8764490-Fresh Water,
pubmed-meshheading:8764490-Genes, Bacterial,
pubmed-meshheading:8764490-Gram-Positive Asporogenous Rods, Irregular,
pubmed-meshheading:8764490-Half-Life,
pubmed-meshheading:8764490-Hot Temperature,
pubmed-meshheading:8764490-Molecular Sequence Data,
pubmed-meshheading:8764490-RNA, Bacterial,
pubmed-meshheading:8764490-RNA, Ribosomal, 16S,
pubmed-meshheading:8764490-Water Microbiology
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pubmed:year |
1996
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pubmed:articleTitle |
A novel thermostable dextranase from a Thermoanaerobacter species cultured from the geothermal waters of the Great Artesian Basin of Australia.
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pubmed:affiliation |
School of Biomolecular and Biomedical Sciences, Faculty of Science and Technology, Griffith University, Nathan Brisbane, Australia.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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