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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-12-10
|
| pubmed:abstractText |
The deposition of complement (C) components on tissues of transplanted organs may induce many proinflammatory responses. The role of such C activation in allograft rejection is uncertain. We addressed this question by inhibiting C at the level of the C3 and C5 convertases, preventing C activation and progression of its cascade, using recombinant human soluble complement receptor 1 (sCR1) in an unsensitized rat renal allograft model. Fully MHC disparate Lewis to DA rat renal allograft recipients given 25 mg/kg sCR1 daily, with saline-treated allograft recipients as controls (n = 15 in each group), were sacrificed from day 1 to day 5 post-transplant, and examined histopathologically, and for the deposition of C3 and C5b-9 membrane attack complex (MAC), and for the presence of leucocyte antigen markers. Treated animals demonstrated a reduction in vascular injury and cellular infiltration, coincident with reduced C deposition. Flow cytometric analysis of leucocyte subpopulations in the spleen showed a reduction in activated (CD25 positive) B and T cells in treated animals, compared to saline treated controls. The results suggest that C inhibition with sCR1, in an unsensitized model of allograft rejection, was able to suppress the vascular and cell mediated components of tissue injury. The data support not only a role for C in antibody and possibly cell mediated cytotoxicity in the graft, but also suggest a role in the primary immune response leading to both T cell and B cell activation.
|
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Mar
|
| pubmed:issn |
0966-3274
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
4
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
72-5
|
| pubmed:dateRevised |
2006-9-28
|
| pubmed:meshHeading |
pubmed-meshheading:8762017-Animals,
pubmed-meshheading:8762017-Flow Cytometry,
pubmed-meshheading:8762017-Graft Rejection,
pubmed-meshheading:8762017-Humans,
pubmed-meshheading:8762017-Kidney,
pubmed-meshheading:8762017-Kidney Transplantation,
pubmed-meshheading:8762017-Rats,
pubmed-meshheading:8762017-Rats, Inbred Lew,
pubmed-meshheading:8762017-Receptors, Complement,
pubmed-meshheading:8762017-Recombinant Proteins,
pubmed-meshheading:8762017-Spleen,
pubmed-meshheading:8762017-T-Lymphocyte Subsets
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
Allograft immune response with sCR1 intervention.
|
| pubmed:affiliation |
Department of Renal Medicine, Guy's Hospital, London, UK.
|
| pubmed:publicationType |
Journal Article
|