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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
7
|
| pubmed:dateCreated |
1996-9-17
|
| pubmed:abstractText |
Chlamydomonas reinhardtii strains harboring deletions of the chloroplast atpB 3' inverted repeat (IR) are weakly phototrophic due to reduced accumulation of discrete atpB transcripts and the chloroplast ATPase beta-subunit protein. A sequence of 18 guanosine residues, which can impede a 3'-->5' exoribonuclease in vitro, is able to substitute for the atpB IR in vivo. Strains containing the poly-guanosine tract in place of the atpB 3' IR are phototrophic and accumulate near wild-type levels of discrete atpB transcripts and the ATPase beta-subunit protein. Because these atpB transcripts contain the 18 guanosine residues, and the poly-guanosine tract is not a terminator of transcription, the accumulation of discrete atpB transcripts is likely the result of impediment of 3'-->5' exoribonuclease activity. These findings support a model in which atpB transcripts lacking the 3' IR are degraded by 3'-->5' exoribonuclease activity, and demonstrate that the poly-guanosine tract can be used to study chloroplast RNA metabolism in vivo.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jul
|
| pubmed:issn |
1355-8382
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
2
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
652-63
|
| pubmed:dateRevised |
2008-11-20
|
| pubmed:meshHeading |
pubmed-meshheading:8756408-Adenosine Triphosphatases,
pubmed-meshheading:8756408-Animals,
pubmed-meshheading:8756408-Base Sequence,
pubmed-meshheading:8756408-Chlamydomonas reinhardtii,
pubmed-meshheading:8756408-Chloroplasts,
pubmed-meshheading:8756408-Escherichia coli,
pubmed-meshheading:8756408-Exoribonucleases,
pubmed-meshheading:8756408-Guanosine,
pubmed-meshheading:8756408-Models, Genetic,
pubmed-meshheading:8756408-Molecular Sequence Data,
pubmed-meshheading:8756408-Photosynthesis,
pubmed-meshheading:8756408-Plasmids,
pubmed-meshheading:8756408-Polydeoxyribonucleotides,
pubmed-meshheading:8756408-RNA Processing, Post-Transcriptional,
pubmed-meshheading:8756408-Repetitive Sequences, Nucleic Acid,
pubmed-meshheading:8756408-Transcription, Genetic,
pubmed-meshheading:8756408-Transformation, Genetic
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A chloroplast transcript lacking the 3' inverted repeat is degraded by 3'-->5' exoribonuclease activity.
|
| pubmed:affiliation |
Boyce Thompson Institute for Plant Research, Cornell University, Ithaca, New York 14853, USA.
|
| pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|