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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
3
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pubmed:dateCreated |
1996-12-10
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pubmed:abstractText |
Connective tissue growth factor (CTGF) is a 36-to 38-kDa peptide that is selectively induced by transforming growth factor-beta (TGF-beta) in fibroblastic cell types. We compared the biologic activities of CTGF with TGF-beta on fibroblasts in culture and in animal models of fibroplasia. CTGF was active as a mitogen in monolayer cultures of normal rat kidney fibroblasts. CTGF did not stimulate anchorage-independent growth of NRK fibroblasts, however, or inhibit the growth of mink lung epithelial cells, distinguishing CTGF's growth-regulatory activities from those of TGF-beta. In NRK fibroblasts, both TGF-beta and CTGF significantly increased the transcripts encoding alpha 1 type I collagen, alpha 5 integrin, and fibronectin. Stimulation of type I collagen and fibronectin protein synthesis by TGF-beta and CTGF was confirmed by pulse labeling of cells with [35S]methionine. Subcutaneous injection of TGF-beta and CTGF into neonatal NIH Swiss mice resulted in a large stimulation of granulation tissue and fibrosis at the site of injection. In situ hybridization studies revealed that TGF-beta injection induced high levels of CTGF mRNA in the dermal fibroblasts at the injection site, demonstrating that TGF-beta can induce the expression of CTGF in connective tissue cells in vivo. No CTGF transcripts were detected in the epidermal cells in either control or TGF-beta-injected skin or in fibroblasts in control (saline-injected) skin. These results demonstrate that, like TGF-beta, CTGF can induce connective tissue cell proliferation and extracellular matrix synthesis.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Connective Tissue Growth Factor,
http://linkedlifedata.com/resource/pubmed/chemical/Ctgf protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Ctgf protein, rat,
http://linkedlifedata.com/resource/pubmed/chemical/Extracellular Matrix Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Inhibitors,
http://linkedlifedata.com/resource/pubmed/chemical/Growth Substances,
http://linkedlifedata.com/resource/pubmed/chemical/Immediate-Early Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Intercellular Signaling Peptides...,
http://linkedlifedata.com/resource/pubmed/chemical/Mitogens,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0022-202X
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
107
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
404-11
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:8751978-Animals,
pubmed-meshheading:8751978-Cell Division,
pubmed-meshheading:8751978-Connective Tissue Growth Factor,
pubmed-meshheading:8751978-Epithelial Cells,
pubmed-meshheading:8751978-Epithelium,
pubmed-meshheading:8751978-Extracellular Matrix,
pubmed-meshheading:8751978-Extracellular Matrix Proteins,
pubmed-meshheading:8751978-Fibroblasts,
pubmed-meshheading:8751978-Granulation Tissue,
pubmed-meshheading:8751978-Growth Inhibitors,
pubmed-meshheading:8751978-Growth Substances,
pubmed-meshheading:8751978-Immediate-Early Proteins,
pubmed-meshheading:8751978-Intercellular Signaling Peptides and Proteins,
pubmed-meshheading:8751978-Mice,
pubmed-meshheading:8751978-Mice, Inbred Strains,
pubmed-meshheading:8751978-Mitogens,
pubmed-meshheading:8751978-RNA, Messenger,
pubmed-meshheading:8751978-Rats,
pubmed-meshheading:8751978-Recombinant Proteins,
pubmed-meshheading:8751978-Skin,
pubmed-meshheading:8751978-Transcription, Genetic,
pubmed-meshheading:8751978-Transforming Growth Factor beta
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pubmed:year |
1996
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pubmed:articleTitle |
Stimulation of fibroblast cell growth, matrix production, and granulation tissue formation by connective tissue growth factor.
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pubmed:affiliation |
Department of Cell Biology and Anatomy, University of Miami School of Medicine, FL 33136, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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