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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0001640,
umls-concept:C0010453,
umls-concept:C0034693,
umls-concept:C0034721,
umls-concept:C0079281,
umls-concept:C0150312,
umls-concept:C0178719,
umls-concept:C0205263,
umls-concept:C0596235,
umls-concept:C0871261,
umls-concept:C1550548,
umls-concept:C1555714,
umls-concept:C1704632,
umls-concept:C1705654,
umls-concept:C1706817,
umls-concept:C2339371,
umls-concept:C2911692
|
| pubmed:issue |
6
|
| pubmed:dateCreated |
1996-10-8
|
| pubmed:abstractText |
Previously we demonstrated that stimulation of cultured neonatal rat ventricular myocytes by either alpha 1-adrenergic agonist or endothelin-1 resulted in a rapid formation of total inositolphosphates, although the levels of inositol 1,4,5-trisphosphate and inositol 1,3,4,5-tetrakisphosphate did not rise significantly. The aim of this study was to examine whether stimulation by alpha 1-adrenergic agonist and endothelin-1 could still elicit phosphatidylinositol cycle mediated intracellular Ca2+ mobilization in these cells. The intracellular free Ca2+ concentration ([Ca2+]i) was measured by single cell imaging dual wavelength fluorescence microscopy in Fura-2-loaded cardiomyocytes. The interference of agonist induced [Ca2+]i responses by the beat to beat variation of [Ca2+]i was prevented by arresting the cells with the Ca2+ entry blocker diltiazem (10 microM). The [Ca2+]i response (expressed as % of baseline ratio of fluorescence intensities of Fura-2 at 340 nm and 380 nm excitation wavelength), induced by phenylephrine (10(-4) M) and endothelin-1 (10(-8) M) was small, up to 20% of baseline after 9-20 min. In contrast, Ca(2+)-influx induced by incubation in Na(+)-free buffer caused a steep increase of [Ca2+]i up to 150% of baseline after 30 s. Analysis of single cells following stimulation with phenylephrine or endothelin-1 showed heterogeneity with respect to a rise in [Ca2+]i. However, if rapid Ca(2+)-influx was induced by incubation in Na(+)-free buffer, [Ca2+]i responses in individual myocytes occurred homogeneously. It is concluded that the alpha 1-adrenergic agonist and endothelin-1 induced [Ca2+]i responses are delayed in time, small and quite heterogeneous among cells. The findings are in agreement with earlier observations which revealed no detectable overall increase of the Ca2+ releasing inositolphosphates under these conditions and suggest that other second messengers, such as 1,2-diacylglycerol, are involved in the agonist mediated Ca2+ signals.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Adrenergic alpha-Agonists,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium,
http://linkedlifedata.com/resource/pubmed/chemical/Calcium Channel Blockers,
http://linkedlifedata.com/resource/pubmed/chemical/Diltiazem,
http://linkedlifedata.com/resource/pubmed/chemical/Endothelins,
http://linkedlifedata.com/resource/pubmed/chemical/Fluorescent Dyes,
http://linkedlifedata.com/resource/pubmed/chemical/Fura-2,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol 1,4,5-Trisphosphate,
http://linkedlifedata.com/resource/pubmed/chemical/Inositol Phosphates,
http://linkedlifedata.com/resource/pubmed/chemical/Phenylephrine,
http://linkedlifedata.com/resource/pubmed/chemical/Sodium,
http://linkedlifedata.com/resource/pubmed/chemical/Tritium,
http://linkedlifedata.com/resource/pubmed/chemical/inositol-1,3,4,5-tetrakisphosphate
|
| pubmed:status |
MEDLINE
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| pubmed:month |
Dec
|
| pubmed:issn |
0143-4160
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
18
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
515-25
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:8746950-Adrenergic alpha-Agonists,
pubmed-meshheading:8746950-Animals,
pubmed-meshheading:8746950-Calcium,
pubmed-meshheading:8746950-Calcium Channel Blockers,
pubmed-meshheading:8746950-Cells, Cultured,
pubmed-meshheading:8746950-Diltiazem,
pubmed-meshheading:8746950-Endothelins,
pubmed-meshheading:8746950-Fluorescent Dyes,
pubmed-meshheading:8746950-Fura-2,
pubmed-meshheading:8746950-Heart Ventricles,
pubmed-meshheading:8746950-Inositol 1,4,5-Trisphosphate,
pubmed-meshheading:8746950-Inositol Phosphates,
pubmed-meshheading:8746950-Microscopy, Fluorescence,
pubmed-meshheading:8746950-Myocardium,
pubmed-meshheading:8746950-Phenylephrine,
pubmed-meshheading:8746950-Rats,
pubmed-meshheading:8746950-Rats, Wistar,
pubmed-meshheading:8746950-Second Messenger Systems,
pubmed-meshheading:8746950-Sodium,
pubmed-meshheading:8746950-Tritium
|
| pubmed:year |
1995
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| pubmed:articleTitle |
Alpha-adrenergic agonist and endothelin-1 induced intracellular Ca2+ response in the presence of a Ca2+ entry blocker in cultured rat ventricular myocytes.
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| pubmed:affiliation |
Department of Biochemistry, Faculty of Medicine and Health Sciences, Erasmus University Rotterdam, The Netherlands.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|