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rdf:type |
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lifeskim:mentions |
umls-concept:C0005767,
umls-concept:C0007634,
umls-concept:C0007961,
umls-concept:C0036679,
umls-concept:C0237868,
umls-concept:C0521457,
umls-concept:C0806140,
umls-concept:C1546426,
umls-concept:C1548280,
umls-concept:C1706211,
umls-concept:C1858460,
umls-concept:C2004454
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pubmed:issue |
2
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pubmed:dateCreated |
1996-9-5
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pubmed:abstractText |
Fetal blood cells can be recovered from the maternal circulation by charge flow separation (CFS), a method that obviates the risks associated with amniocentesis and chorionic villus sampling. By CFS, we processed blood samples from 13 women carrying male fetuses, 2 carrying fetuses with trisomy 21, and 1 who had delivered a stillborn infant with trisomy 18. On average more than 2000 fetal nucleated red blood cells were recovered per 20-ml sample of maternal blood. Recovery of fetal cells was confirmed by fluorescence in situ hybridization with probes for chromosomes Y, 18 and 21. After culturing of CFS-processed cells, amplification by the polymerase chain reaction revealed Y-chromosomal DNA in clones from four of six women bearing male fetuses, but not in clones from three women bearing female fetuses.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:status |
MEDLINE
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pubmed:month |
Aug
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pubmed:issn |
0340-6717
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pubmed:author |
pubmed-author:AddisKK,
pubmed-author:KoeppelRR,
pubmed-author:MüllerU RUR,
pubmed-author:ManleyMM,
pubmed-author:McGavranLL,
pubmed-author:Murata-CollinsJJ,
pubmed-author:ParkerN BNB,
pubmed-author:PhillipsO POP,
pubmed-author:PorrecoRR,
pubmed-author:RuffaloT MTM,
pubmed-author:SammonsDD,
pubmed-author:ShulmanL PLP,
pubmed-author:TaronD JDJ,
pubmed-author:TwittyGG,
pubmed-author:UtermohlenJJ,
pubmed-author:WachtelGG,
pubmed-author:WachtelS SSS
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pubmed:issnType |
Print
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pubmed:volume |
98
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
162-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8698333-Cell Separation,
pubmed-meshheading:8698333-Chromosomes, Human, Pair 18,
pubmed-meshheading:8698333-Down Syndrome,
pubmed-meshheading:8698333-Erythrocytes,
pubmed-meshheading:8698333-Female,
pubmed-meshheading:8698333-Fetal Blood,
pubmed-meshheading:8698333-Humans,
pubmed-meshheading:8698333-In Situ Hybridization, Fluorescence,
pubmed-meshheading:8698333-Male,
pubmed-meshheading:8698333-Pregnancy,
pubmed-meshheading:8698333-Prenatal Diagnosis,
pubmed-meshheading:8698333-Trisomy,
pubmed-meshheading:8698333-Y Chromosome
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pubmed:year |
1996
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pubmed:articleTitle |
Fetal cells in maternal blood: recovery by charge flow separation.
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pubmed:affiliation |
Department of Obstetrics and Gynecology, University of Tennessee, Memphis 38103, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.
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