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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
3
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pubmed:dateCreated |
1996-9-4
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pubmed:abstractText |
Peritoneal macrophages (M phi) constitutively synthesize and secrete interferon-alpha (IFN-alpha) and IFN-beta, as well as complement subcomponent C1q. Because exogenous interferon-gamma (IFN-gamma) stimulates Mø synthesis of C1q, our purpose was to determine if endogenous secretion of IFN-alpha/beta regulated the constitutive level of endogenous C1q mRNA synthesis in an autocrine fashion. Both exogenous IFN-alpha and IFN-beta effectively substituted for IFN-gamma in stimulating M phi C1q mRNA expression in a dose-dependent fashion by northern blot analysis. Neutralizing anti-INF-alpha/beta antibodies inhibited M phi constitutive C1q mRNA synthesis by approximately twofold and abrogated the feedback stimulatory effects of exogenous C1q on C1q mRNA expression. Paraffin oil-elicited inflammatory M phi displayed distinctively different constitutive levels of C1q mRNA expression from thioglycollate brothelicited M phi, which was correlated with their relative levels of secretory IFN-alpha/beta by ELISA. Exogenous IFN-alpha/beta also restored C1q mRNA synthesis of AKR mouse M phi with low constitutive C1q mRNA expression. The cumulative results support the concept that constitutive synthesis of C1q by M phi is regulated by the endogenous synthesis and secretion of IFN-alpha/beta, which appears to act in an autocrine fashion.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Mar
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pubmed:issn |
1079-9907
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
16
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
209-15
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8697143-Animals,
pubmed-meshheading:8697143-Antigen-Antibody Reactions,
pubmed-meshheading:8697143-Base Sequence,
pubmed-meshheading:8697143-Complement C1q,
pubmed-meshheading:8697143-Feedback,
pubmed-meshheading:8697143-Interferon-alpha,
pubmed-meshheading:8697143-Interferon-beta,
pubmed-meshheading:8697143-Macrophages, Peritoneal,
pubmed-meshheading:8697143-Male,
pubmed-meshheading:8697143-Mice,
pubmed-meshheading:8697143-Mice, Inbred AKR,
pubmed-meshheading:8697143-Mice, Inbred C3H,
pubmed-meshheading:8697143-Molecular Sequence Data,
pubmed-meshheading:8697143-RNA, Messenger,
pubmed-meshheading:8697143-Up-Regulation
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pubmed:year |
1996
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pubmed:articleTitle |
Autocrine induction of macrophage synthesis of complement subcomponent C1q by endogenous interferon-alpha/beta.
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pubmed:affiliation |
Oklahoma Medical Research Foundation, Noble Center for Biomedical Research, Oklahoma City 73104-5046, USA.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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