Linked Life Data

8618416

Source:http://linkedlifedata.com/resource/pubmed/id/8618416

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1-2
pubmed:dateCreated
1996-6-11
pubmed:abstractText
A procedure was developed allowing repetitive depletion and recovery of K+ from the cell interior of Müller glial cells without patch pipette perfusion. To this end the whole-cell voltage-clamp technique using tight seal pipettes was applied to enzymatically isolated Müller cells of the guinea pig. When K+ was replaced by Cs+ and/or NMDG in the pipette solution voltage-activated outward currents could be generated similar to those found with normal K(+)-containing intracellular solution. This was the result of the distribution of K+ across the cell membrane due to the negative holding potential, i.e., K+ accumulated at the intracellular side of the cell membrane. This distribution should be favored by the low input resistance of Müller cells. K+ could also be removed from the cell interior by depolarizing voltage steps. Simultaneously, K+ influx had to be cut off by using a K(+)-free extracellular solution or blocking K+ channels using Ba2+. This procedure lead to reduction and eventually cessation of the K+ outward currents, indicating extinction of the intracellular K+ pool. The process is reversible, i.e., outward currents can be evoked again by depolarizing voltage steps after renewed application of extracellular K+ and/or removal of Ba2+. Hence, the intracellular K+ pool is filled up again. The described method was applied to demonstrate the dependence of the Müller cell Na+/glutamate transporter on the intracellular K+ concentration.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:issn
0165-0270
pubmed:author
pubmed:issnType
Print
pubmed:volume
61
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
169-84
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:articleTitle
Repetitive depletion and recovery of intracellular K+ in retinal Müller glial cells during whole-cell voltage-clamp.
pubmed:affiliation
Paul-Flechsig-Institute for Brain Research, Department of Neurophysiology, Leipzig, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't