Linked Life Data

8605676

Source:http://linkedlifedata.com/resource/pubmed/id/8605676

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1996-5-17
pubmed:abstractText
We report here the first RIA for 1,25-dihydroxyvitamin D utilizing a radioiodinated (125I) tracer. This is also the first validated RIA for 1,25-dihydroxyvitamin D [1,25(OH)2D] that does not require sample prepurification by HPLC before the binding assay. The assay involves acetonitrile extraction, treatment of the crude extract supernate with sodium periodate, extraction and purification of endogenous 1,25(OH)2D by solid-phase chromatography, and finally, quantification by RIA. Calibrators were prepared in stripped human serum and processed exactly the same as samples, eliminating the need for internal control for procedural losses of endogenous 1,25(OH)2D. The assay consists of a 2-h room temperature incubation with the primary antibody, a 20-min incubation with a second antibody, and separation of bound from free by centrifugation. Assay results can be in hand with 5 h. The detection limit of the assay is 2.4 ng/L 1,25-dihydroxyvitamin D3. Results compare well with those from an accepted radioreceptor assay. Sample pretreatment with sodium periodate is absolutely essential before quantification by RIA; otherwise, concentrations of endogenous 1,25(OH)2D may be greatly overestimated.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0009-9147
pubmed:author
pubmed:issnType
Print
pubmed:volume
42
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
586-92
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1996
pubmed:articleTitle
Quantification of circulating 1,25-dihydroxyvitamin D by radioimmunoassay with 125I-labeled tracer.
pubmed:affiliation
Department of Pediatrics, Medical University of South Carolina, Charleston, 29425, USA.
pubmed:publicationType
Journal Article, Comparative Study