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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1996-3-1
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pubmed:abstractText |
DNA topoisomerase I (topo I) is involved in the regulation of DNA supercoiling, gene transcription, and rDNA recombination. However, little is known about interactions between topo I and other nuclear proteins. We used affinity chromatography with a topo I fusion protein to screen U-937 leukemic cell extracts and have identified nucleolin as a topo I-binding protein. Coimmunoprecipitation and other studies demonstrate that the interaction between topo I and nucleolin is direct. Furthermore, deletion analyses have identified the 166-210-amino acid region of topo I as sufficient for the interaction with nucleolin. Since nucleolin has been implicated in nuclear transport and in a variety of transcriptional processes, the interaction with topo I may relate to the cellular localization of topo I or to the known role of this topoisomerase in transcription.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
|
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pubmed:status |
MEDLINE
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|
pubmed:month |
Jan
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|
pubmed:issn |
0021-9258
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|
pubmed:author |
|
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pubmed:issnType |
Print
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pubmed:day |
26
|
|
pubmed:volume |
271
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1993-7
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pubmed:dateRevised |
2007-11-14
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pubmed:meshHeading |
pubmed-meshheading:8567649-Amino Acid Sequence,
pubmed-meshheading:8567649-Binding Sites,
pubmed-meshheading:8567649-Cell Compartmentation,
pubmed-meshheading:8567649-DNA, Superhelical,
pubmed-meshheading:8567649-DNA Topoisomerases, Type I,
pubmed-meshheading:8567649-Humans,
pubmed-meshheading:8567649-Molecular Sequence Data,
pubmed-meshheading:8567649-Nuclear Proteins,
pubmed-meshheading:8567649-Phosphoproteins,
pubmed-meshheading:8567649-RNA-Binding Proteins,
pubmed-meshheading:8567649-Sequence Alignment,
pubmed-meshheading:8567649-Sequence Homology, Amino Acid,
pubmed-meshheading:8567649-Tumor Cells, Cultured
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|
pubmed:year |
1996
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|
pubmed:articleTitle |
Identification of a nucleolin binding site in human topoisomerase I.
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pubmed:affiliation |
Division of Cancer Pharmacology, Dana-Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
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pubmed:publicationType |
Journal Article,
Comparative Study,
Research Support, U.S. Gov't, P.H.S.
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