Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
1
|
| pubmed:dateCreated |
1996-3-7
|
| pubmed:databankReference | |
| pubmed:abstractText |
The paternal-effect embryonic-lethal gene, spe-11, is required for normal development of early C. elegans embryos. Spe-11 embryos fail to complete meiosis, form a weak eggshell, fail to orient properly the first mitotic spindle, and fail to undergo cytokinesis. Here we report cloning and sequencing of the spe-11 gene, which encodes a novel protein. As predicted by the paternal-effect mutant phenotype, the gene is expressed during spermatogenesis but is not detectable in females undergoing oogenesis, and the protein is present in mature sperm. To investigate whether SPE-11's essential function is during spermatogenesis or whether sperm-delivered SPE-11 functions in the newly fertilized embryo, we engineered animals to supply SPE-11 to the embryo through the oocyte rather than through the sperm. We found that maternal expression is sufficient for embryonic viability. This result demonstrates that SPE-11 is not required during spermatogenesis, and suggests that SPE-11 is a sperm-supplied factor that participates directly in development of the early embryo. In contrast to the many known maternal factors required for embryogenesis, SPE-11 is the first paternally contributed factor to be genetically identified and molecularly characterized.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Caenorhabditis elegans Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Helminth,
http://linkedlifedata.com/resource/pubmed/chemical/Helminth Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SPE-11 protein, C elegans
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Jan
|
| pubmed:issn |
0950-1991
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
122
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
391-404
|
| pubmed:dateRevised |
2007-11-14
|
| pubmed:meshHeading |
pubmed-meshheading:8565851-Amino Acid Sequence,
pubmed-meshheading:8565851-Animals,
pubmed-meshheading:8565851-Base Sequence,
pubmed-meshheading:8565851-Caenorhabditis elegans,
pubmed-meshheading:8565851-Caenorhabditis elegans Proteins,
pubmed-meshheading:8565851-Cell Nucleus,
pubmed-meshheading:8565851-DNA, Complementary,
pubmed-meshheading:8565851-DNA, Helminth,
pubmed-meshheading:8565851-Female,
pubmed-meshheading:8565851-Gene Expression Regulation, Developmental,
pubmed-meshheading:8565851-Genes, Helminth,
pubmed-meshheading:8565851-Genes, Lethal,
pubmed-meshheading:8565851-Helminth Proteins,
pubmed-meshheading:8565851-Male,
pubmed-meshheading:8565851-Molecular Sequence Data,
pubmed-meshheading:8565851-Nuclear Proteins,
pubmed-meshheading:8565851-Spermatogenesis,
pubmed-meshheading:8565851-Spermatozoa,
pubmed-meshheading:8565851-Zygote
|
| pubmed:year |
1996
|
| pubmed:articleTitle |
A sperm-supplied factor required for embryogenesis in C. elegans.
|
| pubmed:affiliation |
Department of Biology, Indiana University, Bloomington 47405, USA.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.
|