|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
50
|
|
pubmed:dateCreated |
1996-1-26
|
|
pubmed:databankReference |
|
|
pubmed:abstractText |
B cell-specific transcriptional promoter activity mediated by the octamer motif requires the Oct1 or Oct2 protein and additional B cell-restricted cofactors. One such cofactor, BOB.1/OBF.1, was recently isolated from human B cells. Here, we describe the isolation and detailed characterization of the murine homolog. Full-length cDNAs and genomic clones were isolated, and the gene structure was determined. Comparison of the deduced amino acids shows 88% sequence identity between mouse and human BOB.1/OBF.1. The NH2-terminal 126 amino acids of BOB.1/OBF.1 are both essential and sufficient for interaction with the POU domains of either Oct1 or Oct2. This protein-protein interaction does not require the simultaneous binding of Oct proteins to DNA, and high resolution footprinting of the Oct-DNA interaction reveals that binding of BOB.1/OBF.1 to Oct1 or Oct2 does not alter the interaction with DNA. BOB.1/OBF.1 can efficiently activate octamer-dependent promoters in fibroblasts; however, it fails to stimulate octamer-dependent enhancer activity. Fusion of subdomains of BOB.1/OBF.1 with the GAL4 DNA binding domain reveals that both NH2- and COOH-terminal domains of BOB.1/OBF.1 contribute to full transactivation function, the COOH-terminal domain is more efficient in this transactivation assay. Consistent with the failure of full-length BOB.1/OBF.1 to stimulate octamer-dependent enhancer elements in non B cells, the GAL4 fusions likewise only stimulate from a promoter-proximal position.
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA Primers,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HCFC1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Hcfc1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Host Cell Factor C1,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-1,
http://linkedlifedata.com/resource/pubmed/chemical/Octamer Transcription Factor-2,
http://linkedlifedata.com/resource/pubmed/chemical/POU2AF1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/POU2F1 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/POU2F2 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2af1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f1 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Pou2f2 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0021-9258
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:day |
15
|
|
pubmed:volume |
270
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
29870-80
|
|
pubmed:dateRevised |
2008-11-21
|
|
pubmed:meshHeading |
pubmed-meshheading:8530384-Amino Acid Sequence,
pubmed-meshheading:8530384-Animals,
pubmed-meshheading:8530384-B-Lymphocytes,
pubmed-meshheading:8530384-Base Sequence,
pubmed-meshheading:8530384-Cloning, Molecular,
pubmed-meshheading:8530384-DNA Primers,
pubmed-meshheading:8530384-DNA-Binding Proteins,
pubmed-meshheading:8530384-Exons,
pubmed-meshheading:8530384-Homeodomain Proteins,
pubmed-meshheading:8530384-Host Cell Factor C1,
pubmed-meshheading:8530384-Humans,
pubmed-meshheading:8530384-Introns,
pubmed-meshheading:8530384-Mice,
pubmed-meshheading:8530384-Molecular Sequence Data,
pubmed-meshheading:8530384-Mutagenesis, Site-Directed,
pubmed-meshheading:8530384-Octamer Transcription Factor-1,
pubmed-meshheading:8530384-Octamer Transcription Factor-2,
pubmed-meshheading:8530384-Polymerase Chain Reaction,
pubmed-meshheading:8530384-Promoter Regions, Genetic,
pubmed-meshheading:8530384-Restriction Mapping,
pubmed-meshheading:8530384-Substrate Specificity,
pubmed-meshheading:8530384-Trans-Activators,
pubmed-meshheading:8530384-Transcription, Genetic,
pubmed-meshheading:8530384-Transcription Factors
|
|
pubmed:year |
1995
|
|
pubmed:articleTitle |
Functional characterization of the murine homolog of the B cell-specific coactivator BOB.1/OBF.1.
|
|
pubmed:affiliation |
Zentrum für Molekulare Biologie Heidelberg (ZMBH), Federal Republic of Germany.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
