Switch to
Predicate | Object |
---|---|
rdf:type | |
lifeskim:mentions | |
pubmed:issue |
1-2
|
pubmed:dateCreated |
1996-1-23
|
pubmed:abstractText |
The regulation of apolipoprotein A-I (apo A-I) gene expression by 12-O-tetradecanoylphorbol 13-acetate (TPA) was investigated in the human hepatoma cell line Hep G2. TPA treatment decreased apo A-I mRNA levels in a time-dependent manner, by up to 50% versus control cells within 24 h. Nuclear run-on transcription assays demonstrated a transcriptional effect of TPA. Using transfection analysis with a plasmid construct containing the -1378/+11 apo A-I promoter fused to the secreted placental alkaline phosphatase (SPAP) reporter gene, we showed that the SPAP activity was decreased to 50% when Hep G2 cells were incubated in the presence of TPA. The inhibitory effect of TPA was still maintained when fragment -253 to -4 of apo A-I promoter was linked to the CAT reporter gene. These data indicate that transcriptional modulation of apolipoprotein A-I gene expression following phorbol ester treatment is transduced by gene elements located between -253 and -4 of the apo A-I promoter.
|
pubmed:language |
eng
|
pubmed:journal | |
pubmed:citationSubset |
IM
|
pubmed:chemical | |
pubmed:status |
MEDLINE
|
pubmed:month |
Nov
|
pubmed:issn |
0014-5793
|
pubmed:author | |
pubmed:issnType |
Print
|
pubmed:day |
27
|
pubmed:volume |
376
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
99-102
|
pubmed:dateRevised |
2008-11-21
|
pubmed:meshHeading |
pubmed-meshheading:8521977-Apolipoprotein A-I,
pubmed-meshheading:8521977-Base Sequence,
pubmed-meshheading:8521977-Carcinoma, Hepatocellular,
pubmed-meshheading:8521977-Cell Line,
pubmed-meshheading:8521977-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:8521977-Humans,
pubmed-meshheading:8521977-Molecular Sequence Data,
pubmed-meshheading:8521977-Promoter Regions, Genetic,
pubmed-meshheading:8521977-Tetradecanoylphorbol Acetate,
pubmed-meshheading:8521977-Transcription, Genetic,
pubmed-meshheading:8521977-Transfection
|
pubmed:year |
1995
|
pubmed:articleTitle |
Transcriptional regulation of apolipoprotein A-I expression in Hep G2 cells by phorbol ester.
|
pubmed:affiliation |
CNRS URA 1283, CHU Saint Antoine, Paris, France.
|
pubmed:publicationType |
Journal Article
|