| pubmed-article:8506294 | rdf:type | pubmed:Citation | lld:pubmed |
| pubmed-article:8506294 | lifeskim:mentions | umls-concept:C0024264 | lld:lifeskim |
| pubmed-article:8506294 | lifeskim:mentions | umls-concept:C1281743 | lld:lifeskim |
| pubmed-article:8506294 | lifeskim:mentions | umls-concept:C1527148 | lld:lifeskim |
| pubmed-article:8506294 | lifeskim:mentions | umls-concept:C1510438 | lld:lifeskim |
| pubmed-article:8506294 | lifeskim:mentions | umls-concept:C0314627 | lld:lifeskim |
| pubmed-article:8506294 | pubmed:issue | 10 | lld:pubmed |
| pubmed-article:8506294 | pubmed:dateCreated | 1993-7-2 | lld:pubmed |
| pubmed-article:8506294 | pubmed:abstractText | We describe a system to evaluate the function of lymphocyte-specific and generally expressed genes in the differentiation and/or function of lymphocytes. RAG-2 (recombination-activating gene 2)-deficient mice have no mature B and T lymphocytes due to the inability to initiate VDJ recombination. Blastocysts from RAG-2-deficient mice generate animals with no mature B and T cells following implantation into foster mothers. However, injection of normal ES cells into RAG-2-deficient blastocysts leads to the generation of somatic chimeras with mature B and T cells all of which derive from the injected ES cells (referred to as RAG-2-deficient blastocyst complementation). Complementation of RAG-2-deficient blastocysts with mutant ES cells heterozygous for a targeted mutation that deletes all immunoglobulin heavy-chain joining (JH) gene segments (JH+/-) also leads to generation of chimeras with normal B and T cells. However, complementation with ES cells homozygous for the JH mutation (JH-/-) generates animals with normal T cells but no B cells, due to a block in B-cell development at a very early stage. Transfection of a functionally assembled mu heavy-chain gene into the JH-/- ES cells prior to blastocyst injection rescues the JH-/- mutation and allows the generation of both mature T and mature B cells. The rescued B cells express IgM but not IgD and respond normally to bacterial lipopolysaccharide stimulation by proliferating and by secreting IgM. | lld:pubmed |
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| pubmed-article:8506294 | pubmed:language | eng | lld:pubmed |
| pubmed-article:8506294 | pubmed:journal | http://linkedlifedata.com/r... | lld:pubmed |
| pubmed-article:8506294 | pubmed:citationSubset | IM | lld:pubmed |
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| pubmed-article:8506294 | pubmed:status | MEDLINE | lld:pubmed |
| pubmed-article:8506294 | pubmed:month | May | lld:pubmed |
| pubmed-article:8506294 | pubmed:issn | 0027-8424 | lld:pubmed |
| pubmed-article:8506294 | pubmed:author | pubmed-author:YuHH | lld:pubmed |
| pubmed-article:8506294 | pubmed:author | pubmed-author:ChenJJ | lld:pubmed |
| pubmed-article:8506294 | pubmed:author | pubmed-author:YoungFF | lld:pubmed |
| pubmed-article:8506294 | pubmed:author | pubmed-author:StewardFF | lld:pubmed |
| pubmed-article:8506294 | pubmed:author | pubmed-author:LansfordRR | lld:pubmed |
| pubmed-article:8506294 | pubmed:issnType | Print | lld:pubmed |
| pubmed-article:8506294 | pubmed:day | 15 | lld:pubmed |
| pubmed-article:8506294 | pubmed:volume | 90 | lld:pubmed |
| pubmed-article:8506294 | pubmed:owner | NLM | lld:pubmed |
| pubmed-article:8506294 | pubmed:authorsComplete | Y | lld:pubmed |
| pubmed-article:8506294 | pubmed:pagination | 4528-32 | lld:pubmed |
| pubmed-article:8506294 | pubmed:dateRevised | 2009-11-18 | lld:pubmed |
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| pubmed-article:8506294 | pubmed:year | 1993 | lld:pubmed |
| pubmed-article:8506294 | pubmed:articleTitle | RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development. | lld:pubmed |
| pubmed-article:8506294 | pubmed:affiliation | Howard Hughes Medical Institute, Children's Hospital, Department of Genetics, Harvard University Medical School, Boston. | lld:pubmed |
| pubmed-article:8506294 | pubmed:publicationType | Journal Article | lld:pubmed |
| pubmed-article:8506294 | pubmed:publicationType | Research Support, U.S. Gov't, P.H.S. | lld:pubmed |
| pubmed-article:8506294 | pubmed:publicationType | Research Support, Non-U.S. Gov't | lld:pubmed |
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