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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1993-7-2
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pubmed:abstractText |
We describe a system to evaluate the function of lymphocyte-specific and generally expressed genes in the differentiation and/or function of lymphocytes. RAG-2 (recombination-activating gene 2)-deficient mice have no mature B and T lymphocytes due to the inability to initiate VDJ recombination. Blastocysts from RAG-2-deficient mice generate animals with no mature B and T cells following implantation into foster mothers. However, injection of normal ES cells into RAG-2-deficient blastocysts leads to the generation of somatic chimeras with mature B and T cells all of which derive from the injected ES cells (referred to as RAG-2-deficient blastocyst complementation). Complementation of RAG-2-deficient blastocysts with mutant ES cells heterozygous for a targeted mutation that deletes all immunoglobulin heavy-chain joining (JH) gene segments (JH+/-) also leads to generation of chimeras with normal B and T cells. However, complementation with ES cells homozygous for the JH mutation (JH-/-) generates animals with normal T cells but no B cells, due to a block in B-cell development at a very early stage. Transfection of a functionally assembled mu heavy-chain gene into the JH-/- ES cells prior to blastocyst injection rescues the JH-/- mutation and allows the generation of both mature T and mature B cells. The rescued B cells express IgM but not IgD and respond normally to bacterial lipopolysaccharide stimulation by proliferating and by secreting IgM.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1329091,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1406933,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1409620,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1459450,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1547487,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1547488,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1557357,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1569957,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1605908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1622894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1827140,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1838007,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1910681,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-1913803,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-2065352,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-2215662,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-2360047,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-2598259,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-2694945,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-3043226,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-3194019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-3261841,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-3317825,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-6086308,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-6336590,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-6788376,
http://linkedlifedata.com/resource/pubmed/commentcorrection/8506294-8458340
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
May
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
90
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4528-32
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:8506294-Animals,
pubmed-meshheading:8506294-B-Lymphocytes,
pubmed-meshheading:8506294-Blastocyst,
pubmed-meshheading:8506294-Cell Differentiation,
pubmed-meshheading:8506294-DNA-Binding Proteins,
pubmed-meshheading:8506294-Female,
pubmed-meshheading:8506294-Gene Rearrangement, B-Lymphocyte, Heavy Chain,
pubmed-meshheading:8506294-Genes, Immunoglobulin,
pubmed-meshheading:8506294-Genetic Complementation Test,
pubmed-meshheading:8506294-Immunoglobulin mu-Chains,
pubmed-meshheading:8506294-Mice,
pubmed-meshheading:8506294-Mice, Mutant Strains,
pubmed-meshheading:8506294-Proteins,
pubmed-meshheading:8506294-Restriction Mapping,
pubmed-meshheading:8506294-T-Lymphocytes
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pubmed:year |
1993
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pubmed:articleTitle |
RAG-2-deficient blastocyst complementation: an assay of gene function in lymphocyte development.
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pubmed:affiliation |
Howard Hughes Medical Institute, Children's Hospital, Department of Genetics, Harvard University Medical School, Boston.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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