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Predicate | Object |
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rdf:type | |
lifeskim:mentions |
umls-concept:C0018134,
umls-concept:C0024297,
umls-concept:C0033268,
umls-concept:C0035647,
umls-concept:C0035696,
umls-concept:C0202202,
umls-concept:C0205178,
umls-concept:C0220781,
umls-concept:C0237400,
umls-concept:C0441889,
umls-concept:C0591833,
umls-concept:C1515655,
umls-concept:C1533691,
umls-concept:C1705241,
umls-concept:C1705242,
umls-concept:C1706089,
umls-concept:C1883254
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pubmed:issue |
4
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pubmed:dateCreated |
1993-6-23
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pubmed:abstractText |
The in vivo activation of lymphokine-producing cells was analyzed in the first 7 days of an acute graft-versus-host reaction (GVHR) induced by injection of C57BL/6 spleen cells into irradiated DBA/2 mice. Although the GVHR was accompanied by a 1000-fold increase in serum IL-6 titers, circulating levels of other lymphokines were low (IL-3, IFN-gamma, and GM-CSF) or undetectable (IL-2 and IL-4). Spleen and lymph node cells from these mice nevertheless produced elevated levels of IL-3, IFN-gamma, GM-CSF, and IL-6 when cultured for 24 h without stimulation; culture with anti-CD3 antibody further increased IL-2, IL-3, IL-4, IFN-gamma, and GM-CSF production by at least 20-fold. Both constitutive and anti-CD3-induced synthesis of all the lymphokines was mediated by CD3+ cells. Messenger RNA analyses revealed the presence of IL-6, IFN-gamma, and GM-CSF transcripts in freshly harvested GVHR spleen cells and increased expression of IL-2, IL-3, IL-4, IFN-gamma, and GM-CSF mRNAs following anti-CD3 stimulation in vitro. In vivo, however, IL-3 mRNA was barely detectable even following cDNA amplification by polymerase chain reaction. In vivo restimulation of day 5 GVHR mice by injection of concanavalin A enhanced expression of IL-3, IL-6, IFN-gamma, and GM-CSF mRNAs and markedly increased serum titers of the corresponding lymphokines, which peaked 6-12 h after injection at levels at least 10- to 100-fold higher than in concanavalin A-treated normal mice.2+ for high-level synthesis of all these lymphokines.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical | |
pubmed:status |
MEDLINE
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pubmed:month |
Apr
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pubmed:issn |
0953-8178
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
399-407
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:8494826-Animals,
pubmed-meshheading:8494826-Concanavalin A,
pubmed-meshheading:8494826-Gene Expression Regulation,
pubmed-meshheading:8494826-Graft vs Host Reaction,
pubmed-meshheading:8494826-Lymphokines,
pubmed-meshheading:8494826-Mice,
pubmed-meshheading:8494826-Mice, Inbred C57BL,
pubmed-meshheading:8494826-Mice, Inbred DBA,
pubmed-meshheading:8494826-RNA, Messenger,
pubmed-meshheading:8494826-T-Lymphocytes
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pubmed:year |
1993
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pubmed:articleTitle |
Lymphokine synthesis in vivo in an acute murine graft-versus-host reaction: mRNA and protein measurements in vivo and in vitro reveal marked differences between actual and potential lymphokine production levels.
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pubmed:affiliation |
Walter and Eliza Hall Institute of Medical Research, Royal Melbourne Hospital, Victoria, Australia.
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pubmed:publicationType |
Journal Article,
Comparative Study,
In Vitro,
Research Support, Non-U.S. Gov't
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